Effect of L-methioninyl adenylate on the level of aminoacylation in vivo of tRNA(Met) from Escherichia coli K12.

Nucleic acids research ·Vol. 1 ·No. 5 ·1974-05-00 ·Pages 719-25

Cassio D, Mathien Y

Abstract

In cells of E.coli K12 grown exponentially in minimal medium, tRNA(met), tRNA(leu) and tRNA(ile) are aminoacylated at 100%, 80% and 64%, respectively. On addition of L-methioninyl adenylate to the growth medium, one observes a specific deacylation of tRNA(met). When more than 35% of tRNA(met) is deacylated, growth rate is reduced and becomes proportional to the amount of methionyl-tRNA formed.

MeSH Terms

Adenosine Monophosphate/analogs & derivatives,pharmacology Escherichia coli/drug effects,growth & development,metabolism Methionine/analogs & derivatives,pharmacology RNA, Bacterial/metabolism RNA, Transfer, Amino Acyl/metabolism RNA, Transfer, Ile/metabolism RNA, Transfer, Leu/metabolism RNA, Transfer, Met/metabolism

Chemicals

RNA, Bacterial RNA, Transfer, Amino Acyl RNA, Transfer, Ile RNA, Transfer, Leu RNA, Transfer, Met methioninyl adenylate Adenosine Monophosphate Methionine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cassio D

Mathien Y

References (10)

10 references, click to expand

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Effect of methioninyl adenylate on the growth of E. coli K 12.
FEBS Lett. 1973 Sep 1;35(1):112-6 PMID: 4584624
Regulation of histidyl-transfer ribonucleic acid synthetase formation in a histidyl-transfer ribonucleic acid synthetase mutant of Salmonella typhimurium.
J Bacteriol. 1972 Sep;111(3):739-44 PMID: 4559825
Purification and properties of methionyl-tRNA synthetase from E. coli K 12 carrying the F32 episome.
FEBS Lett. 1971 Feb 9;12(6):309-312 PMID: 11945606
Biochemical and physiological properties of methionyl-sRNA synthetase mutants of Salmonella typhimurium.
J Gen Microbiol. 1971 Jan;65(1):5-21 PMID: 4326110
Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo.
J Bacteriol. 1970 Apr;102(1):204-12 PMID: 4908672
Regulation of methionyl-transfer ribonucleic acid synthetase formation in Escherichia coli and Salmonella typhimurium.
J Bacteriol. 1973 Jun;114(3):1007-13 PMID: 4576394
Reversible specific concentration of amino acids in Escherichia coli.
Ann Inst Pasteur (Paris). 1956 Nov;91(5):693-720 PMID: 13395009
DEMONSTRATION OF AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE IN A MUTANT OF ESCHERICHIA COLI.
J Biol Chem. 1964 Jun;239:1839-43 PMID: 14213362
Histidine regulation in Salmonella typhimurium. XI. The percentage of transfer RNA His charged in vivo and its relation to the repression of the histidine operon.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.5.719

Published

1974-05-00

Pages

719-25

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343372

Subset

External Links

PubMed source DOI: 10.1093/nar/1.5.719 PMC Full Text View journal details

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Effect of L-methioninyl adenylate on the level of aminoacylation in vivo of tRNA(Met) from Escherichia coli K12.

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