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PMID: 8180021 Published · ppublish English Journal Article

Expression of transforming growth factor alpha, amphiregulin and cripto-1 in human breast carcinomas.

British journal of cancer ·Vol. 69 ·No. 5 ·1994-05-00 ·Pages 903-10

Qi CF, Liscia DS, Normanno N, Merlo G, Johnson GR, Gullick WJ, Ciardiello F, Saeki T, Brandt R, Kim N

Abstract

The expression of three epidermal growth factor (EGF)-related peptides, transforming growth factor alpha (TGF-alpha), amphiregulin (AR) and cripto-1 (CR-1), was examined by immunocytochemistry (ICC) in 68 primary infiltrating ductal (IDCs) and infiltrating lobular breast carcinomas (ILCs), and in 23 adjacent non-involved human mammary tissue samples. Within the 68 IDC and ILC specimens, 54 (79%) expressed immunoreactive TGF-alpha, 52 (77%) expressed AR and 56 (82%) expressed CR-1. Cytoplasmic staining was observed with all of the antibodies, and this staining could be eliminated by preabsorption of the antibodies with the appropriate peptide immunogen. Cytoplasmic staining with all of the antibodies was confined to the carcinoma cells, since no specific immunoreactivity could be detected in the surrounding stromal or endothelial cells. In addition to cytoplasmic reactivity, the AR antibody also exhibited nuclear staining in a number of the carcinoma specimens. No significant correlations were found between the percentage of carcinoma cells that were positive for TGF-alpha, AR or CR-1 and oestrogen receptor status, axillary lymph node involvement, histological grade, tumour size, proliferative index, loss of heterozygosity on chromosome 17p or overall patient survival. However, a highly significant inverse correlation was observed between the average percentage of carcinoma cells that expressed AR in individual tumours and the presence of a point-mutated p53 gene. Likewise, a significantly higher percentage of tumour cells in the ILC group expressed AR as compared with the average percentage of tumour cells that expressed AR in the IDC group. Of the 23 adjacent, non-involved breast tissue samples, CR-1 could be detected by ICC in only three (13%), while TGF-alpha was found in six (26%) and AR in ten (43%) of the non-involved breast tissues. These data demonstrate that breast carcinomas express multiple EGF-related peptides and show that the differential expression of CR-1 in malignant breast epithelial cells may serve as a potential tumour marker for breast cancer.

MeSH Terms
Amphiregulin Biomarkers, Tumor/analysis Breast Neoplasms/chemistry EGF Family of Proteins Epidermal Growth Factor Female GPI-Linked Proteins Genes, p53/genetics Glycoproteins/analysis Growth Substances/analysis Humans Intercellular Signaling Peptides and Proteins Membrane Glycoproteins Neoplasm Proteins/analysis Point Mutation/genetics RNA, Messenger/analysis RNA, Neoplasm/analysis Transforming Growth Factor alpha/analysis
Chemicals
AREG protein, human Amphiregulin Biomarkers, Tumor EGF Family of Proteins GPI-Linked Proteins Glycoproteins Growth Substances Intercellular Signaling Peptides and Proteins Membrane Glycoproteins Neoplasm Proteins RNA, Messenger RNA, Neoplasm TDGF1 protein, human Transforming Growth Factor alpha Epidermal Growth Factor
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Qi C F
Tumor Growth Factor Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Liscia D S
Normanno N
Merlo G
Johnson G R
Gullick W J
Ciardiello F
Saeki T
Brandt R
Kim N
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1994.174
Published
1994-05-00
Pages
903-10
Language
English
Region
England
NLM ID
0370635
PMCID
PMC1968887
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1994.174 PMC Full Text View journal details
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