Home LiteratureArticle Details

PMID: 8152916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of high titre helper-free recombinant retroviral vectors by lipofection.

Nucleic acids research ·Vol. 22 ·No. 6 ·1994-03-25 ·Pages 1117-8

Fassati A, Takahara Y, Walsh FS, Dickson G

Abstract

暂无摘要

MeSH Terms

3T3 Cells Animals Cation Exchange Resins Genetic Vectors Lipids Mice Retroviridae/genetics Transfection/methods beta-Galactosidase/genetics

Chemicals

Cation Exchange Resins Lipids Lipofectamine beta-Galactosidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fassati A

Department of Experimental Pathology, U.M.D.S. Guy's Hospital, London, UK.

Takahara Y

Walsh F S

Dickson G

References (8)

8 references, click to expand

Factors involved in production of helper virus-free retrovirus vectors.
Somat Cell Mol Genet. 1986 Mar;12(2):175-83 PMID: 3457477
Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):156-60 PMID: 3099292
A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for beta-galactosidase.
Biotechniques. 1989 Jun;7(6):576-9 PMID: 2517211
Production of high-titer helper-free retroviruses by transient transfection.
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8392-6 PMID: 7690960
A new retrovirus packaging cell for gene transfer constructed from amplified long terminal repeat-free chimeric proviral genes.
J Virol. 1992 Jun;66(6):3725-32 PMID: 1316479
Packaging system for rapid production of murine leukemia virus vectors with variable tropism.
J Virol. 1992 Aug;66(8):5110-3 PMID: 1321291
Recent advances in retrovirus vector technology.
Curr Opin Genet Dev. 1993 Feb;3(1):102-9 PMID: 8453267
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.
Nucleic Acids Res. 1990 Jun 25;18(12):3587-96 PMID: 2194165

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1994-03-25

Pages

1117-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC307939

Subset

External Links

Analysis Services