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PMID: 8144453 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region.

Journal of bacteriology ·Vol. 176 ·No. 7 ·1994-04-00 ·Pages 1872-80

Barton AB, Kaback DB

Abstract

Transcribed regions on a 42-kb segment of chromosome I from Saccharomyces cerevisiae were mapped. Polyadenylated transcripts corresponding to eight previously characterized genes (MAK16, LTE1, CCR4, FUN30, FUN31, TPD3, DEP1, and CYS3) and eight new genes were identified. All transcripts were present at one to four copies per cell except for one which was significantly less abundant. This region has been sequenced, and the sizes, locations, and orientations of the transcripts were in nearly perfect agreement with the open reading frames. Disruptions in eight genes identified solely on the basis of a transcribed region, FUN38, FUN25, FUN26, FUN28, FUN30, FUN31, FUN33, and FUN34, indicated that all were nonessential for growth on rich medium at 30 degrees C. Disruption of FUN30, a gene closely related to RAD16 and RAD54, surprisingly resulted in increased resistance to UV irradiation. No additional phenotypes, other than slow growth, were observed for all other mutants. The distribution of essential genes on chromosome I is discussed.

Related Genes
FUN 25 FUN 26 FUN 28 FUN 30 FUN 31 FUN 33 FUN 34
MeSH Terms
Chromosome Mapping Chromosomes, Fungal Cloning, Molecular DNA Mutational Analysis Dose-Response Relationship, Radiation Gene Library Genes, Fungal/genetics Mutagenesis, Insertional RNA, Messenger/genetics Radiation Tolerance/genetics Saccharomyces cerevisiae/genetics,radiation effects Sequence Analysis, DNA Sequence Deletion Transcription, Genetic Ultraviolet Rays/adverse effects
Chemicals
RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barton A B
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Kaback D B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.7.1872-1880.1994
Published
1994-04-00
Pages
1872-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205289
Subset
IM
Grants
NHGRI NIH HHS · HG00800 · United States
External Links
PubMed source DOI: 10.1128/jb.176.7.1872-1880.1994 PMC Full Text View journal details
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