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PMID: 2192864 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A.

The EMBO journal ·Vol. 9 ·No. 7 ·1990-07-00 ·Pages 2321-9

Heyer WD, Rao MR, Erdile LF, Kelly TJ, Kolodner RD

Abstract

Single-stranded DNA binding proteins (SSBs) are known to play a role in DNA replication and recombination in prokaryotes. An SSB was previously purified from the yeast Saccharomyces cerevisiae. This SSB stimulated the activity of a cognate strand exchange protein (SEP1) in vitro suggesting a role in recombination. We have cloned and functionally analyzed the gene encoding this protein. DNA sequencing of the cloned DNA revealed a 621 amino acid open reading frame with a coding potential for a Mr 70,269 polypeptide. Highly significant amino acid homology was detected between this S.cerevisiae gene and the Mr 70,000 subunit polypeptide of human RP-A, a cellular protein essential for SV40 DNA replication in vitro. Therefore, we named the S.cerevisiae gene RPA1. RPA1 encodes an essential function in this organism as shown by tetrad analysis of heterozygous insertion mutants and is continuously required for mitotic growth. Cells lacking RPA1 accumulate as multiply budded cells with a single nucleus suggesting a defect in DNA replication.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Fungal Cloning, Molecular Crosses, Genetic DNA-Binding Proteins/genetics Fungal Proteins/genetics Gene Library Genes, Fungal Genotype Humans Macromolecular Substances Molecular Sequence Data Molecular Weight Plasmids Replication Protein A Restriction Mapping Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid
Chemicals
DNA-Binding Proteins Fungal Proteins Macromolecular Substances RPA1 protein, human RPP1A protein, S cerevisiae Replication Protein A Ribosomal Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Heyer W D
Laboratory of Molecular Genetics, Dana-Farber Cancer Institute, Boston, MA 02115.
Rao M R
Erdile L F
Kelly T J
Kolodner R D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-07-00
Pages
2321-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551959
Subset
IM
Grants
NIGMS NIH HHS · GM29383 · United States
NIGMS NIH HHS · GM42780 · United States
Databases
GENBANK
M31528, M60262
External Links
PubMed source PMC Full Text View journal details
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