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PMID: 8134392 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and structure of a pre-B-cell growth-stimulating factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 6 ·1994-03-15 ·Pages 2305-9

Nagasawa T, Kikutani H, Kishimoto T

Abstract

Generation and proliferation of early B-cell progenitors have been known to require stromal cell-derived molecules. A stromal cell line, PA6, was found to produce a soluble mediator, which was distinct from interleukin 7 (IL-7) and stem cell factor and supported the proliferation of a stromal cell-dependent pre-B-cell clone, DW34. A cDNA clone encoding this DW34 growth-stimulating factor was isolated by expression cloning. The nucleotide sequence contained a single substantial open reading frame of 267 nucleotides encoding an 89-amino acid polypeptide. The amino acid sequence of this cytokine, designated pre-B-cell growth-stimulating factor (PBSF), revealed that it is a member of intercrine alpha subfamily. Recombinant PBSF stimulated the proliferation of DW34 cells for itself and, furthermore, synergistically augmented the growth of DW34 as well as bone marrow B-cell progenitors in the presence of IL-7.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/cytology,immunology Base Sequence Blotting, Northern Bone Marrow Cells Cell Differentiation/physiology Cell Line Chemokine CXCL12 Chemokines, CXC Cloning, Molecular Culture Media Cytokines/genetics,physiology DNA Mice Mice, Inbred BALB C Molecular Sequence Data Stromal Cells/cytology Transfection
Chemicals
Chemokine CXCL12 Chemokines, CXC Culture Media Cxcl12 protein, mouse Cytokines DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nagasawa T
Division of Immunology, Osaka University, Japan.
Kikutani H
Kishimoto T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.6.2305
Published
1994-03-15
Pages
2305-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43359
Subset
IM
Databases
GENBANK
D21072
External Links
PubMed source DOI: 10.1073/pnas.91.6.2305 PMC Full Text View journal details
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