Home LiteratureArticle Details
PMID: 1827140 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow.

The Journal of experimental medicine ·Vol. 173 ·No. 5 ·1991-05-01 ·Pages 1213-25

Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K

Abstract

We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others, expressing S7, include B lineage cells before pre-B. These S7+ fractions, provisionally termed Fr. A, Fr. B, and Fr. C, can differentiate in a stromal layer culture system. Phenotypic alteration during such culture suggests an ordering of these stages from Fr. A to Fr. B to Fr. C and thence to S7- pre-B cells. Using polymerase chain reaction amplification with pairs of oligonucleotide primers for regions 5' of JH1, DFL16.1, and Jk1, we find that the Ig genes of Fr. A are in germline configuration, whereas Fr. B and C are pro-B cell stages with increasing D-J rearrangement, but no V-D-J. Finally, functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr. A.

MeSH Terms
Animals Antigens, CD Antigens, Surface/genetics,immunology,metabolism B-Lymphocytes/cytology,immunology,metabolism Base Sequence Biotin/metabolism Bone Marrow/immunology,metabolism Bone Marrow Cells Cell Differentiation/physiology Cell Membrane/metabolism,ultrastructure Cells, Cultured DNA/analysis,genetics Female Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics,immunology Gene Rearrangement, B-Lymphocyte, Light Chain/genetics,immunology Hematopoietic Stem Cells/cytology,immunology,metabolism Immunoglobulin Heavy Chains/genetics,immunology,metabolism Leukosialin Mice Molecular Sequence Data Peptide Fragments/genetics,immunology,metabolism Phenotype Phycoerythrin/metabolism Polymerase Chain Reaction Sialoglycoproteins/metabolism
Chemicals
Antigens, CD Antigens, Surface Immunoglobulin Heavy Chains Leukosialin Peptide Fragments Sialoglycoproteins Spn protein, mouse Phycoerythrin Biotin DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hardy R R
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Carmack C E
Shinton S A
Kemp J D
Hayakawa K
References (46)
46 references, click to expand
  1. Unusual immunoglobulin DNA sequences from the nonexpressed chromosome of mouse normal B lymphocytes: implications for allelic exclusion and the DNA rearrangement process.
    J Immunol. 1987 Sep 1;139(5):1718-26 PMID: 3114375
  2. J genes for heavy chain immunoglobulins of mouse.
    Science. 1980 Sep 5;209(4461):1128-32 PMID: 6250219
  3. Induction of aggregation and enhancement of proliferation and IL-2 secretion in human T cells by antibodies to CD43.
    J Immunol. 1988 Nov 1;141(9):2912-7 PMID: 3049818
  4. Use of a monoclonal antibody (GA3) to demonstrate lineage restricted O-glycosylation on leukosialin during terminal erythroid differentiation.
    Blood. 1988 May;71(5):1226-33 PMID: 2451948
  5. Expression and functional significance of the J11d marker on mouse thymocytes.
    J Immunol. 1987 Apr 1;138(7):2013-8 PMID: 2435787
  6. Developmentally controlled and tissue-specific expression of unrearranged VH gene segments.
    Cell. 1985 Feb;40(2):271-81 PMID: 2578321
  7. Mechanism of mononuclear cell activation by an anti-CD43 (sialophorin) agonistic antibody.
    J Immunol. 1989 Jun 15;142(12):4194-200 PMID: 2542404
  8. Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16.
    Proc Natl Acad Sci U S A. 1989 Feb;86(4):1328-32 PMID: 2521952
  9. Identification of a defective mouse immunoglobulin D (diversity) element which can undergo DJH, but not VHD, recombination.
    Immunogenetics. 1989;30(5):383-6 PMID: 2509341
  10. Only DFL16, DSP2, and DQ52 gene families exist in mouse immunoglobulin heavy chain diversity gene loci, of which DFL16 and DSP2 originate from the same primordial DH gene.
    Eur J Immunol. 1989 Oct;19(10):1849-54 PMID: 2583225
  11. The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule.
    J Immunol. 1989 Nov 15;143(10):3303-8 PMID: 2809203
  12. Interleukin 7 production and function in stromal cell-dependent B cell development.
    J Exp Med. 1989 Jul 1;170(1):333-8 PMID: 2787384
  13. A monoclonal antibody to sialophorin (CD43) induces homotypic adhesion and activation of human monocytes.
    J Exp Med. 1989 Jul 1;170(1):259-67 PMID: 2787380
  14. Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2819-23 PMID: 2784859
  15. Normal B cell precursors responsive to recombinant murine IL-7 and inhibition of IL-7 activity by transforming growth factor-beta.
    J Immunol. 1989 Jun 1;142(11):3875-83 PMID: 2785567
  16. Identification and characterization of a mouse cell surface antigen with alternative molecular forms.
    Immunogenetics. 1988;28(5):295-302 PMID: 3169878
  17. How immunoglobulin V kappa genes rearrange.
    J Immunol. 1987 Dec 1;139(11):3834-9 PMID: 3119721
  18. Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4634-8 PMID: 2660142
  19. Phosphorylation of the major leukocyte surface sialoglycoprotein, leukosialin, is increased by phorbol 12-myristate 13-acetate.
    J Biol Chem. 1989 Nov 5;264(31):18824-31 PMID: 2530225
  20. A single VH gene is utilized predominantly in anti-BrMRBC hybridomas derived from purified Ly-1 B cells. Definition of the VH11 family.
    J Immunol. 1989 May 15;142(10):3643-51 PMID: 2497178
  21. The sequence of rat leukosialin (W3/13 antigen) reveals a molecule with O-linked glycosylation of one third of its extracellular amino acids.
    EMBO J. 1987 Dec 20;6(13):4029-34 PMID: 2965006
  22. Relationships between B-lineage lymphocytes and stromal cells in long-term bone marrow cultures.
    Eur J Immunol. 1987 Oct;17(10):1473-84 PMID: 2960538
  23. A novel cell surface molecule on early B-lineage cells.
    Nature. 1986 Jun 5-11;321(6070):616-8 PMID: 3086746
  24. Regulation of the assembly and expression of variable-region genes.
    Annu Rev Immunol. 1986;4:339-68 PMID: 3085692
  25. Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation.
    J Immunol. 1988 Jun 1;140(11):3751-7 PMID: 2897394
  26. Differentiation of B cell progenitors in vitro: generation of surface IgM+ B cells, including Ly-1 B cells, from Thy-1- asialoGM1+ cells in newborn liver.
    Eur J Immunol. 1987 Dec;17(12):1769-74 PMID: 2891538
  27. Stimulation of B-cell progenitors by cloned murine interleukin-7.
    Nature. 1988 Jun 9;333(6173):571-3 PMID: 3259677
  28. B lymphocyte precursors and myeloid progenitors survive in diffusion chamber cultures but B cell differentiation requires close association with stromal cells.
    Blood. 1987 Nov;70(5):1418-24 PMID: 3499188
  29. Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule.
    Cell. 1987 Mar 27;48(6):1009-21 PMID: 3493849
  30. Differential expression of a surface antigen recognized by a monoclonal antibody, J11d, on unprimed and primed B cells.
    J Immunol. 1986 Aug 1;137(3):791-7 PMID: 3487587
  31. Sialophorin, a surface sialoglycoprotein defective in the Wiskott-Aldrich syndrome, is involved in human T lymphocyte proliferation.
    J Exp Med. 1987 May 1;165(5):1383-92 PMID: 3572301
  32. Regulated progression of a cultured pre-B-cell line to the B-cell stage.
    Nature. 1985 Sep 26-Oct 2;317(6035):353-5 PMID: 3930970
  33. Identification of early stages of T lymphocyte development in the thymus cortex and medulla.
    J Immunol. 1985 Jun;134(6):3632-42 PMID: 3886788
  34. BP-1/6C3 expression defines a differentiation stage of transformed pre-B cells and is not related to malignant potential.
    J Immunol. 1990 Sep 1;145(5):1603-8 PMID: 1974567
  35. Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):993-7 PMID: 1689065
  36. Separate elements control DJ and VDJ rearrangement in a transgenic recombination substrate.
    EMBO J. 1990 Jan;9(1):117-25 PMID: 2153073
  37. Expression cloning of a cDNA encoding M1/69-J11d heat-stable antigens.
    J Immunol. 1990 Sep 15;145(6):1952-9 PMID: 2118158
  38. Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.
    Mol Cell Biol. 1983 Jul;3(7):1317-32 PMID: 6412070
  39. Hematopoietic subpopulations express cross-reactive, lineage-specific molecules detected by monoclonal antibody.
    Mol Immunol. 1984 Jun;21(6):507-14 PMID: 6611488
  40. Identification of D segments of immunoglobulin heavy-chain genes and their rearrangement in T lymphocytes.
    Nature. 1981 Apr 16;290(5807):565-70 PMID: 6783962
  41. Aberrant rearrangements contribute significantly to the allelic exclusion of immunoglobulin gene expression.
    Nature. 1981 Apr 2;290(5805):372-8 PMID: 6783959
  42. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
    Immunol Rev. 1982;69:5-23 PMID: 6984001
  43. A monoclonal antibody discriminating between subsets of T and B cells.
    J Immunol. 1981 Dec;127(6):2496-501 PMID: 6975326
  44. Rearrangement and selection of VH11 in the Ly-1 B cell lineage.
    J Exp Med. 1990 Jul 1;172(1):371-4 PMID: 2358782
  45. Ordered rearrangement of immunoglobulin heavy chain variable region segments.
    EMBO J. 1984 Jun;3(6):1209-19 PMID: 6086308
  46. Glycobiology.
    Annu Rev Biochem. 1988;57:785-838 PMID: 3052290
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.5.1213
Published
1991-05-01
Pages
1213-25
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118850
Subset
IM
Grants
NIAID NIH HHS · AI-26782 · United States
NCI NIH HHS · CA-06927 · United States
NCRR NIH HHS · RR-05539 · United States
External Links
PubMed source DOI: 10.1084/jem.173.5.1213 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2