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PMID: 8132475 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid.

Journal of bacteriology ·Vol. 176 ·No. 6 ·1994-03-00 ·Pages 1773-6

Holtel A, Marqués S, Möhler I, Jakubzik U, Timmis KN

Abstract

TOL plasmid-encoded degradation of benzyl alcohol by Pseudomonas putida is inhibited by glucose and other compounds related to the main carbohydrate metabolism in Pseudomonas species. We report here that this effect is exerted at the level of expression of the xyl catabolic operons, and two xyl promoters, Pu and Ps, were identified as the primary targets of this inhibition. xyl promoter activation was also inhibited by glucose in the heterologous Escherichia coli system, apparently not however by the classical mechanism of enteric catabolite repression.

Related Genes
xyl
MeSH Terms
Base Sequence Benzyl Alcohol Benzyl Alcohols/metabolism Escherichia coli Gene Expression Regulation, Bacterial Hydrocarbons/metabolism Models, Genetic Molecular Sequence Data Mutation Operon/genetics,physiology Plasmids/genetics Promoter Regions, Genetic/physiology Pseudomonas putida/genetics,metabolism
Chemicals
Benzyl Alcohols Hydrocarbons Benzyl Alcohol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holtel A
Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Federal Republic of Germany.
Marqués S
Möhler I
Jakubzik U
Timmis K N
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.6.1773-1776.1994
Published
1994-03-00
Pages
1773-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205268
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.6.1773-1776.1994 PMC Full Text View journal details
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