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PMID: 8127661 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning the Drosophila homolog of the xeroderma pigmentosum complementation group C gene reveals homology between the predicted human and Drosophila polypeptides and that encoded by the yeast RAD4 gene.

Nucleic acids research ·Vol. 22 ·No. 3 ·1994-02-11 ·Pages 257-61

Henning KA, Peterson C, Legerski R, Friedberg EC

Abstract

A human xeroderma pigmentosum group C (XPC) cDNA has been previously isolated by functional complementation (Legerski and Peterson, Nature, 359, 70-73, 1992). Sequence analysis did not reveal protein motifs which might suggest a possible biochemical function for the putative XPC protein. In order to identify functional domains in the translated XPC sequence the homologous gene from Drosophila melanogaster, designated XPCDM, was cloned by DNA hybridization. Sequence analysis of an apparently full-length cDNA revealed an open reading frame which can encode a predicted polypeptide of 1293 amino acids. Significant homology of the C-terminal 346 amino acids with both the human XPC and Saccharomyces cerevisiae Rad4 protein sequences is observed, suggesting that these proteins are functional homologs.

Related Genes

RAD4 XPC XPC<up>DM</up>

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins Drosophila melanogaster/genetics Fungal Proteins/genetics Genes, Fungal Genes, Insect Humans Molecular Sequence Data Nuclear Proteins/chemistry,genetics Restriction Mapping Saccharomyces cerevisiae/genetics Schizosaccharomyces pombe Proteins Sequence Alignment Sequence Homology, Amino Acid Transglutaminases Xeroderma Pigmentosum/genetics

Chemicals

DNA, Complementary DNA-Binding Proteins Fungal Proteins Nuclear Proteins RAD4 protein, S pombe Schizosaccharomyces pombe Proteins Transglutaminases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Henning K A

Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.

Peterson C

Legerski R

Friedberg E C

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21 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/22.3.257

Published

1994-02-11

Pages

257-61

Language

English

Region

England

NLM ID

0411011

PMCID

PMC523574

Subset

Grants

NCI NIH HHS · CA44247 · United States

NCI NIH HHS · CA52461 · United States

Databases

GENBANK

Z28622

External Links

PubMed source DOI: 10.1093/nar/22.3.257 PMC Full Text View journal details

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Cloning the Drosophila homolog of the xeroderma pigmentosum complementation group C gene reveals homology between the predicted human and Drosophila polypeptides and that encoded by the yeast RAD4 gene.

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