Home LiteratureArticle Details
PMID: 8122395 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of the collagen-like putative oncoprotein of Herpesvirus saimiri in transformed T cells.

Virus genes ·Vol. 7 ·No. 4 ·1993-12-00 ·Pages 349-65

Medveczky MM, Geck P, Vassallo R, Medveczky PG

Abstract

Herpesvirus saimiri induces acute lymphomas and leukemias in New World primates and rabbits. Previous work revealed that a highly oncogenic group C strain 484-77 encodes and expresses a bicistronic mRNA in tumor-derived T cells and the first open reading frame (orf1) is highly homologous to collagen. With the aid of an antibody against a synthetic orf1 peptide, we now report that the orf1 collagen-like protein is expressed in rabbit tumor derived cell lines and in vitro transformed human and monkey T cells. The orf1 protein is expressed in vivo, as indicated by specific antibodies detected in the serum from a tumor-bearing rabbit.

MeSH Terms
Amino Acid Sequence Animals Cell Line, Transformed Collagen/biosynthesis Culture Techniques Fluorescent Antibody Technique Herpesvirus 2, Saimiriine/genetics Humans Molecular Sequence Data Oncogene Proteins/biosynthesis Open Reading Frames Precipitin Tests Protein Biosynthesis Rabbits Transcription, Genetic Viral Proteins/biosynthesis
Chemicals
Oncogene Proteins Viral Proteins Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Medveczky M M
Department of Medical Microbiology and Immunology, University of South Florida, Tampa 33612-4799.
Geck P
Vassallo R
Medveczky P G
References (37)
37 references, click to expand
  1. Structure of nonintegrated, circular Herpesvirus saimiri and Herpesvirus ateles genomes in tumor cell lines and in vitro-transformed cells.
    J Virol. 1982 Oct;44(1):295-310 PMID: 6292492
  2. Identification and characterization of the herpesvirus saimiri oncoprotein STP-C488.
    J Virol. 1991 Dec;65(12):6953-60 PMID: 1658384
  3. In vitro immortalization of marmoset cells with three subgroups of herpesvirus saimiri.
    J Virol. 1987 Nov;61(11):3485-90 PMID: 2822956
  4. Primary structure of the herpesvirus saimiri genome.
    J Virol. 1992 Aug;66(8):5047-58 PMID: 1321287
  5. Biochemical characterization of VLA-1 and VLA-2. Cell surface heterodimers on activated T cells.
    J Biol Chem. 1985 Dec 5;260(28):15246-52 PMID: 2415516
  6. Herpesvirus saimiri: VII. Induction of malignant lymphoma in New Zealand white rabbits.
    J Natl Cancer Inst. 1974 Dec;53(6):1803-7 PMID: 4530878
  7. Structural analysis of p28 adult T-cell leukaemia-associated antigen.
    J Gen Virol. 1986 Jul;67 ( Pt 7):1373-9 PMID: 3014050
  8. Herpesvirus-transformed cytotoxic T-cell lines.
    Nature. 1981 May 7;291(5810):81-3 PMID: 6262648
  9. Four novel U RNAs are encoded by a herpesvirus.
    Cell. 1988 Aug 26;54(5):599-607 PMID: 2842058
  10. Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits.
    J Virol. 1989 Sep;63(9):3601-11 PMID: 2547988
  11. Inverse correlation of collagen production to anchorage independence and tumorigenicity in W8- and M-cell lines.
    Cancer Res. 1983 Sep;43(9):4275-82 PMID: 6871864
  12. Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
    EMBO J. 1986 Dec 20;5(13):3547-52 PMID: 3549279
  13. Attenuation of herpesvirus saimiri for marmosets after successive passage in cell culture at 39 degrees C.
    J Natl Cancer Inst. 1975 Nov;55(5):1243-6 PMID: 173864
  14. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  15. IL-2 independent growth and cytotoxicity of herpesvirus saimiri-infected human CD8 cells and involvement of two open reading frame sequences of the virus.
    Virology. 1993 Oct;196(2):402-12 PMID: 8396794
  16. Deletion of DNA sequence in a nononcogenic variant of Herpesvirus saimiri.
    J Virol. 1984 May;50(2):662-5 PMID: 6323769
  17. Identification of normal and transformed lymphocyte subsets of nonhuman primates with monoclonal antibodies to human lymphocytes.
    J Immunol. 1983 Mar;130(3):1323-9 PMID: 6296233
  18. Low molecular weight fibroblast collagen: structure, secretion, and differential expression as a function of fetal and cellular age.
    Biochemistry. 1985 Dec 3;24(25):7430-40 PMID: 4084589
  19. Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation.
    J Virol. 1989 Aug;63(8):3307-14 PMID: 2545905
  20. Herpesvirus saimiri DNA in a lymphoid cell line established by in vitro transformation.
    J Virol. 1984 Mar;49(3):938-46 PMID: 6321774
  21. Fibronectin and its receptors.
    Annu Rev Biochem. 1988;57:375-413 PMID: 2972252
  22. A single gene and a pseudogene for the cellular tumour antigen p53.
    Nature. 1983 Dec 8-14;306(5943):594-7 PMID: 6646235
  23. Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts.
    Nature. 1978 Apr 13;272(5654):622-4 PMID: 565469
  24. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype.
    Virology. 1990 Jun;176(2):505-14 PMID: 2161148
  25. Herpesvirus saimiri. II. Experimentally induced malignant lymphoma in primates.
    Lab Anim Care. 1969 Jun;19(3):378-86 PMID: 4240129
  26. Structure of Herpesvirus saimiri genomes: arrangement of heavy and light sequences in the M genome.
    J Virol. 1976 Jul;19(1):154-61 PMID: 181593
  27. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  28. An apparently new herpesvirus from primary kidney cultures of the squirrel monkey (Saimiri sciureus).
    Lab Anim Care. 1968 Jun;18(3):374-81 PMID: 4233372
  29. Classification of herpesvirus saimiri into three groups based on extreme variation in a DNA region required for oncogenicity.
    J Virol. 1984 Dec;52(3):938-44 PMID: 6092721
  30. Collagen synthesis by murine bone marrow cell culture.
    J Cell Physiol. 1986 Jun;127(3):397-402 PMID: 3011814
  31. Expression of collagenlike sequences by a tumor virus, herpesvirus saimiri.
    J Virol. 1990 Jul;64(7):3509-15 PMID: 2161952
  32. Herpesviridae. Definition, provisional nomenclature, and taxonomy. The Herpesvirus Study Group, the International Committee on Taxonomy of Viruses.
    Intervirology. 1981;16(4):201-17 PMID: 7343541
  33. Transformation-enhancing activity of gelatin-binding fragments of fibronectin.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4965-9 PMID: 6458038
  34. Stimulation of DNA synthesis by cathepsin D digests of fibronectin.
    Nature. 1983 Oct 27-Nov 2;305(5937):811-3 PMID: 6633653
  35. LFA-1 membrane molecule in the regulation of homotypic adhesions of human B lymphocytes.
    J Immunol. 1985 Jul;135(1):9-11 PMID: 3889159
  36. Comparative studies of collagen lattice contraction utilizing a normal and a transformed cell line.
    J Cell Physiol. 1983 Aug;116(2):159-66 PMID: 6863399
  37. Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7051-5 PMID: 1651491
Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF01703391
Published
1993-12-00
Pages
349-65
Language
English
Region
United States
NLM ID
8803967
Subset
IM
Grants
NIAID NIH HHS · AI 21129 · United States
NCI NIH HHS · R01 CA43264 · United States
NCRR NIH HHS · SO7RR05749 · United States
External Links
PubMed source DOI: 10.1007/BF01703391 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2