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PMID: 8120090 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A triggered mechanism retrieves membrane in seconds after Ca(2+)-stimulated exocytosis in single pituitary cells.

The Journal of cell biology ·Vol. 124 ·No. 5 ·1994-03-00 ·Pages 667-75

Thomas P, Lee AK, Wong JG, Almers W

Abstract

In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test for faster retrieval mechanisms, we have triggered short bursts of exocytosis by flash photolysis of caged Ca2+, and have tracked subsequent retrieval by measuring the plasma membrane capacitance. We find that a limited amount of membrane can be retrieved with a time constant of 4 s at 21-26 degrees C, and that this occurs partially via structures larger than coated vesicles. This novel mechanism may be arrested at a late step. Incomplete retrieval structures then remain on the cell surface for minutes until the consequences of a renewed increase in cytosolic [Ca2+] disconnect them from the cell surface in < 1 s. Our results provide evidence for a rapid, triggered membrane retrieval pathway in excitable cells.

MeSH Terms
Animals Calcium/metabolism Cell Membrane/physiology Cells, Cultured Cytosol/metabolism Endocytosis/physiology Exocytosis/drug effects,physiology Kinetics Models, Biological Photolysis Pituitary Gland/drug effects,physiology Rats Tetraethylammonium Tetraethylammonium Compounds/pharmacology Time Factors
Chemicals
Tetraethylammonium Compounds Tetraethylammonium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thomas P
Department of Physiology and Biophysics, University of Washington, Seattle 98195.
Lee A K
Wong J G
Almers W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.124.5.667
Published
1994-03-00
Pages
667-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119962
Subset
IM
Grants
NIAMS NIH HHS · AR-17803 · United States
NIGMS NIH HHS · T32 GM07270 · United States
External Links
PubMed source DOI: 10.1083/jcb.124.5.667 PMC Full Text View journal details
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