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PMID: 8108438 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic actin structures stabilized by profilin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 4 ·1994-02-15 ·Pages 1510-4

Finkel T, Theriot JA, Dise KR, Tomaselli GF, Goldschmidt-Clermont PJ

Abstract

We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

MeSH Terms
Actins/drug effects,metabolism,ultrastructure Animals CHO Cells/cytology Cell Compartmentation Clone Cells/cytology Contractile Proteins Cricetinae Dose-Response Relationship, Drug Fluorescent Antibody Technique Humans Microfilament Proteins/biosynthesis,genetics,pharmacology Profilins Recombinant Proteins/pharmacology Transfection
Chemicals
Actins Contractile Proteins Microfilament Proteins PFN1 protein, human Profilins Recombinant Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Finkel T
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Theriot J A
Dise K R
Tomaselli G F
Goldschmidt-Clermont P J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.4.1510
Published
1994-02-15
Pages
1510-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43189
Subset
IM
Grants
NIAID NIH HHS · R37 AI036929 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.4.1510 PMC Full Text View journal details
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