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PMID: 810659 Published · ppublish English Journal Article

Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.

Molecular & general genetics : MGG ·Vol. 137 ·No. 4 ·1975-00-00 ·Pages 289-304

Svenningsen BA

Abstract

The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.

MeSH Terms
Aldehyde-Lyases/analysis Cell-Free System Chromosome Mapping Chromosomes, Bacterial DNA, Bacterial/analysis Deoxyribose Escherichia coli/enzymology Genes, Regulator Glucosephosphates Operon Pentosephosphates Phosphotransferases/analysis Purine-Nucleoside Phosphorylase/analysis Ribosemonophosphates Thymidine Phosphorylase/analysis
Chemicals
DNA, Bacterial Glucosephosphates Pentosephosphates Ribosemonophosphates Deoxyribose Purine-Nucleoside Phosphorylase Thymidine Phosphorylase Phosphotransferases Aldehyde-Lyases deoxyribose-phosphate aldolase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Svenningsen B A
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00703255
Published
1975-00-00
Pages
289-304
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00703255
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