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PMID: 4322261 Published · ppublish English Journal Article

An adenosine 3':5'-cyclic monophosphate-binding protein that acts on the transcription process.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 1 ·1971-01-00 ·Pages 215-8

Eron L, Arditti R, Zubay G, Connaway S, Beckwith JR

Abstract

An assay system for the in vitro transcription of the lac operon is described. A protein factor (CAP) and cyclic AMP, which are essential for the lac expression in vivo, also stimulate lac transcription in vitro.

MeSH Terms
Adenine Nucleotides/metabolism Bacterial Proteins/metabolism Coliphages/metabolism Cyclic AMP/metabolism DNA, Viral/metabolism Genes, Regulator Genetic Code Hybridization, Genetic In Vitro Techniques Lactose/metabolism Mutation Operon RNA Nucleotidyltransferases/metabolism RNA, Viral/metabolism Templates, Genetic
Chemicals
Adenine Nucleotides Bacterial Proteins DNA, Viral RNA, Viral Cyclic AMP RNA Nucleotidyltransferases Lactose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Eron L
Arditti R
Zubay G
Connaway S
Beckwith J R
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.1.215
Published
1971-01-00
Pages
215-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391198
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.1.215 PMC Full Text View journal details
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