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PMID: 8105472 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 19 ·1993-10-01 ·Pages 9181-5

Patel N, Thierry-Mieg D, Mancillas JR

Abstract

An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.

Related Genes
dpy-17 e2281 unc-104 unc-116 unc-86
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Caenorhabditis elegans/genetics,metabolism Chromosome Mapping Cloning, Molecular DNA/isolation & purification,metabolism DNA, Complementary Genetic Linkage Genetic Markers Humans Kinesins/biosynthesis,chemistry Macromolecular Substances Molecular Sequence Data Mutagenesis, Insertional Polymorphism, Restriction Fragment Length Protein Structure, Secondary Sequence Homology, Amino Acid
Chemicals
DNA, Complementary Genetic Markers Macromolecular Substances DNA Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Patel N
Molecular Biology Institute, University of California, Los Angeles 90024.
Thierry-Mieg D
Mancillas J R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.19.9181
Published
1993-10-01
Pages
9181-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47526
Subset
IM
Grants
NIGMS NIH HHS · GM 07185 · United States
Databases
GENBANK
L19120
External Links
PubMed source DOI: 10.1073/pnas.90.19.9181 PMC Full Text View journal details
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