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PMID: 2537252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Tc3 family of transposable genetic elements in Caenorhabditis elegans.

Genetics ·Vol. 121 ·No. 1 ·1989-01-00 ·Pages 47-55

Collins J, Forbes E, Anderson P

Abstract

We describe genetic and molecular properties of Tc3, a family of transposable elements in Caenorhabditis elegans. About 15 Tc3 elements are present in the genomes of several different wild-type varieties of C. elegans, but Tc3 transposition and excision are not detected in these strains. Tc3 transposition and excision occur at high frequencies, however, in strain TR679, a mutant identified because of its highly active Tc1 elements. In TR679, Tc3 is responsible for several spontaneous mutations affecting the unc-22 gene. Tc3-induced mutations are unstable, and revertants result from precise or nearly precise excision of Tc3. Although Tc3 is very active in TR679, it is not detectably active in several other mutator mutants, all of which exhibit high levels of Tc1 activity. Tc3 is 2.5 kilobases long, and except for sequences near its inverted repeat termini, it is unrelated to Tc1. The termini of Tc3 are inverted repeats of at least 70 base pairs; the terminal 8 nucleotides of Tc3 are identical to 8 of the terminal 9 nucleotides of Tc1.

MeSH Terms
Animals Base Sequence Caenorhabditis/genetics,isolation & purification Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics Molecular Sequence Data Multigene Family Mutation Nucleic Acid Hybridization Restriction Mapping
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collins J
Department of Genetics, University of Wisconsin, Madison 53706.
Forbes E
Anderson P
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/121.1.47
Published
1989-01-00
Pages
47-55
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203604
Subset
IM
Grants
NIGMS NIH HHS · GM30132 · United States
External Links
PubMed source DOI: 10.1093/genetics/121.1.47 PMC Full Text View journal details
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