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PMID: 8099628 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus type 1 DNA synthesis, integration, and efficient viral replication in growth-arrested T cells.

Journal of virology ·Vol. 67 ·No. 7 ·1993-07-00 ·Pages 3969-77

Li G, Simm M, Potash MJ, Volsky DJ

Abstract

Human immunodeficiency virus type 1 (HIV-1) replicates efficiently in nonproliferating monocytes and macrophages but not in resting primary T lymphocytes. To determine the contribution of cell division to the HIV-1 replicative cycle in T cells, we evaluated HIV-1 expression, integration of proviral DNA, and production of infectious progeny virus in C8166 T-lymphoid cells blocked in cell division by treatment with either mitomycin, a DNA cross-linker, or aphidicolin, a DNA polymerase alpha inhibitor. The arrest of cell division was confirmed by assay of [3H]thymidine uptake; the nondividing cells remained viable for at least 3 days after treatment. HIV-1 was expressed and replicated equally well in nondividing and dividing C8166 cells, as judged by the comparison of the levels of p24 core antigens in culture supernatants, the proportion of cells expressing HIV-1 specific antigens, the pattern and quantity of HIV-1 DNA present in the extrachromosomal and total cellular DNA fractions, and the biological activity of progeny viruses. A polymerase chain reaction-based viral DNA integration assay indicated that HIV-1 provirus was integrated in C8166 cells treated with either of the two inhibitors of cell division. Similar results were obtained by using growth-arrested Jurkat T-lymphoid cells. We conclude that cell division and cellular DNA synthesis are not required for efficient HIV-1 expression in T cells.

MeSH Terms
Aphidicolin/pharmacology Base Sequence CD4-Positive T-Lymphocytes/cytology,microbiology Cells, Cultured Growth Inhibitors HIV-1/growth & development,metabolism Humans In Vitro Techniques Mitomycins/pharmacology Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction Virus Integration Virus Replication
Chemicals
Growth Inhibitors Mitomycins Oligodeoxyribonucleotides Aphidicolin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li G
Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, New York, New York.
Simm M
Potash M J
Volsky D J
References (63)
63 references, click to expand
  1. Cellular functions are required for the synthesis and integration of avian sarcoma virus-specific DNA.
    Cell. 1977 Jun;11(2):307-19 PMID: 196759
  2. Inhibition of viral DNA synthesis in stationary chicken embryo fibroblasts infected with avian retroviruses.
    J Virol. 1977 Nov;24(2):461-9 PMID: 916025
  3. The synthesis and structure of visna virus DNA.
    Virology. 1979 Mar;93(2):377-86 PMID: 222046
  4. Cell cycle dependence of synthesis of unintegrated viral DNA in mouse cells newly infected with murine leukemia virus.
    Virology. 1981 Apr 15;110(1):202-7 PMID: 7210505
  5. Fv-1 host restriction of Friend leukemia virus: analysis of unintegrated proviral DNA.
    J Virol. 1981 Oct;40(1):45-55 PMID: 7288927
  6. Visna DNA synthesis and the tempo of infection in vitro.
    Virology. 1982 Jun;119(2):399-410 PMID: 6281982
  7. Establishment of infection by spleen necrosis virus: inhibition in stationary cells and the role of secondary infection.
    J Virol. 1982 Jan;41(1):183-91 PMID: 6283112
  8. Restricted expression of human T-cell leukemia--lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes.
    Virology. 1983 Aug;129(1):51-64 PMID: 6412453
  9. Active nuclear import of human immunodeficiency virus type 1 preintegration complexes.
    Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6580-4 PMID: 1631159
  10. Lack of human immunodeficiency virus type 1 (HIV-1) replication and accumulation of viral DNA in HIV-1-infected T cells blocked in cell replication.
    J Gen Virol. 1992 Apr;73 ( Pt 4):933-9 PMID: 1634879
  11. Human immunodeficiency virus infection of cells arrested in the cell cycle.
    EMBO J. 1992 Aug;11(8):3053-8 PMID: 1322294
  12. Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells.
    J Virol. 1992 Dec;66(12):7414-9 PMID: 1433523
  13. Integration is essential for efficient gene expression of human immunodeficiency virus type 1.
    J Virol. 1993 Mar;67(3):1169-74 PMID: 8437208
  14. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA.
    Cancer. 1965 Apr;18:522-9 PMID: 14278051
  15. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
    J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PMID: 6606682
  16. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
  17. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.
    J Immunol. 1984 Jul;133(1):123-8 PMID: 6327821
  18. Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes.
    Science. 1984 Jul 6;225(4657):59-63 PMID: 6328660
  19. An attempt to assess the overall diversity of murine T cells using two-dimensional gel electrophoresis.
    Eur J Immunol. 1984 Sep;14(9):769-77 PMID: 6332735
  20. Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.
    Science. 1984 Dec 7;226(4679):1165-71 PMID: 6095449
  21. Trans-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat.
    Science. 1985 Jan 11;227(4683):171-3 PMID: 2981427
  22. Sequence homology and morphologic similarity of HTLV-III and visna virus, a pathogenic lentivirus.
    Science. 1985 Jan 11;227(4683):173-7 PMID: 2981428
  23. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  24. Cellular tropism of the human retrovirus HTLV-III/LAV. I. Role of T cell activation and expression of the T4 antigen.
    J Immunol. 1985 Nov;135(5):3151-62 PMID: 2995487
  25. Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
    Science. 1986 Mar 28;231(4745):1549-53 PMID: 3006244
  26. Infection of monocyte/macrophages by human T lymphotropic virus type III.
    J Clin Invest. 1986 May;77(5):1712-5 PMID: 2422213
  27. In vitro infection of human monocytes with human T lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV).
    J Immunol. 1986 Jul 1;137(1):323-9 PMID: 3011909
  28. Prevalence of AIDS-associated retrovirus and antibodies among male homosexuals at risk for AIDS in Greenwich Village.
    AIDS Res. 1984-1985;1(6):407-21 PMID: 6085926
  29. Human T lymphotropic virus type III infection of human alveolar macrophages.
    Blood. 1986 Jul;68(1):281-4 PMID: 3013342
  30. The role of mononuclear phagocytes in HTLV-III/LAV infection.
    Science. 1986 Jul 11;233(4760):215-9 PMID: 3014648
  31. A human T-cell line resistant to cytopathic effects of the human immunodeficiency virus (HIV).
    Virology. 1987 Jan;156(1):40-9 PMID: 3492810
  32. Retroviral etiology of the acquired immune deficiency syndrome (AIDS).
    AIDS Res. 1986 Dec;2 Suppl 1:S35-48 PMID: 2881556
  33. Pathogenesis of visna/maedi and caprine arthritis-encephalitis: new leads on the mechanism of restricted virus replication and persistent inflammation.
    Vet Immunol Immunopathol. 1987 May;15(1-2):167-80 PMID: 3039719
  34. Persistent productive infection of human glial cells by human immunodeficiency virus (HIV) and by infectious molecular clones of HIV.
    J Virol. 1987 Dec;61(12):3774-82 PMID: 2446007
  35. Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes.
    J Exp Med. 1988 Apr 1;167(4):1428-41 PMID: 3258626
  36. Cytokines alter production of HIV-1 from primary mononuclear phagocytes.
    Science. 1988 Sep 23;241(4873):1673-5 PMID: 3047875
  37. Differences in cytopathogenicity and host cell range among infectious molecular clones of human immunodeficiency virus type 1 simultaneously isolated from an individual.
    J Virol. 1988 Nov;62(11):4078-85 PMID: 3172338
  38. Unexpectedly high levels of HIV-1 RNA and protein synthesis in a cytocidal infection.
    Science. 1988 Dec 16;242(4885):1554-7 PMID: 3201245
  39. Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone.
    J Immunol. 1989 Jan 15;142(2):431-8 PMID: 2463307
  40. Evidence that T cell activation is required for HIV-1 entry in CD4+ lymphocytes.
    J Immunol. 1989 Feb 1;142(3):773-80 PMID: 2521508
  41. Biology and pathogenesis of lentiviruses.
    J Gen Virol. 1989 Jul;70 ( Pt 7):1617-39 PMID: 2544657
  42. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4.
    Science. 1989 Jul 21;245(4915):305-8 PMID: 2665081
  43. HIV-1 replication is controlled at the level of T cell activation and proviral integration.
    EMBO J. 1990 May;9(5):1551-60 PMID: 2184033
  44. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure.
    Cell. 1990 Apr 20;61(2):213-22 PMID: 2331748
  45. Differences in the basal activity of the long terminal repeat determine different replicative capacities of two closely related human immunodeficiency virus type 1 isolates.
    J Virol. 1990 Aug;64(8):3654-60 PMID: 2370677
  46. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.
    Mol Cell Biol. 1990 Aug;10(8):4239-42 PMID: 2370865
  47. Titration of human immunodeficiency virus type 1 (HIV-1) and quantitative analysis of virus expression in vitro using liquid RNA-RNA hybridization.
    J Virol Methods. 1990 Jun;28(3):257-71 PMID: 2200801
  48. HIV DNA integration: observations and interferences.
    J Acquir Immune Defic Syndr. 1990;3(9):839-51 PMID: 2166782
  49. Frequent identification of HIV-1 DNA in bronchoalveolar lavage cells obtained from individuals with the acquired immunodeficiency syndrome.
    Am Rev Respir Dis. 1991 Apr;143(4 Pt 1):850-4 PMID: 1848969
  50. Human immunodeficiency virus type 1 NL4-3 replication in four T-cell lines: rate and efficiency of entry, a major determinant of permissiveness.
    J Virol. 1991 Jul;65(7):3900-2 PMID: 1674969
  51. In vitro maturation of mononuclear phagocytes and susceptibility to HIV-1 infection.
    J Acquir Immune Defic Syndr. 1991;4(8):751-9 PMID: 1856787
  52. Productive human immunodeficiency virus type 1 (HIV-1) infection of nonproliferating human monocytes.
    J Exp Med. 1991 Dec 1;174(6):1477-82 PMID: 1720811
  53. Increased susceptibility of differentiated mononuclear phagocytes to productive infection with human immunodeficiency virus-1 (HIV-1).
    J Clin Invest. 1992 Jan;89(1):176-83 PMID: 1370293
  54. Differential HIV replication and HIV-induced interferon production in mononuclear phagocytes: relationship to cell maturation.
    Res Virol. 1991 Sep-Oct;142(5):353-61 PMID: 1722899
  55. Incompletely reverse-transcribed human immunodeficiency virus type 1 genomes in quiescent cells can function as intermediates in the retroviral life cycle.
    J Virol. 1992 Mar;66(3):1717-25 PMID: 1371173
  56. Synthesis of human immunodeficiency virus DNA in a cell-to-cell transmission model.
    AIDS Res Hum Retroviruses. 1992 Feb;8(2):253-9 PMID: 1540411
  57. Proliferation-dependent HIV-1 infection of monocytes occurs during differentiation into macrophages.
    J Clin Invest. 1992 Apr;89(4):1154-60 PMID: 1556179
  58. Ovine lentivirus is macrophagetropic and does not replicate productively in T lymphocytes.
    J Virol. 1992 May;66(5):2679-88 PMID: 1348546
  59. Nonrandom integration of retroviral DNA in vitro: effect of CpG methylation.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5532-6 PMID: 1319063
  60. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  61. Immunofluorescent localization of equine infectious anemia virus in tissue.
    Am J Pathol. 1971 Feb;62(2):283-94 PMID: 4322275
  62. Cell cycle-dependent activation of rous sarcoma virus-infected stationary chicken cells: avian leukosis virus group-specific antigens and ribonucleic acid.
    J Virol. 1972 Jul;10(1):82-7 PMID: 4339198
  63. The slow infection caused by visna virus.
    Curr Top Microbiol Immunol. 1975;72:101-56 PMID: 56255
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.7.3969-3977.1993
Published
1993-07-00
Pages
3969-77
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237764
Subset
IM
Grants
NCI NIH HHS · CA37465 · United States
NHLBI NIH HHS · HL43628 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.7.3969-3977.1993 PMC Full Text View journal details
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