Home LiteratureArticle Details
PMID: 8095334 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neural expression and chromosomal mapping of Neu differentiation factor to 8p12-p21.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 5 ·1993-03-01 ·Pages 1867-71

Orr-Urtreger A, Trakhtenbrot L, Ben-Levy R, Wen D, Rechavi G, Lonai P, Yarden Y

Abstract

Neu differentiation factor (NDF/heregulin) is a 44-kDa glycoprotein that interacts with the Neu/ErbB-2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. In vitro NDF promotes differentiation of certain mammary tumor cell lines to milk-producing cells. As a first step toward understanding the physiological role of NDF, we performed in situ hybridization analyses to determine mRNA distribution in the mouse embryo and to map the gene to human karyotypes. In 14.5-day-postcoitum mouse embryos, NDF expression is confined predominantly to the central and peripheral nervous system, including the neuroepithelium that lines the lateral ventricles of the brain, the ventral horn of the spinal cord, and the intestinal as well as dorsal root ganglia. Other tissues that contain NDF transcripts are the adrenal gland, liver, and distinct cell layers of the dermis and germinal ridge. In situ hybridization of a 3H-labeled probe to human metaphase spreads localized the NDF gene to the short arm of chromosome 8 at bands p12-p21.

Related Genes
neu
MeSH Terms
Animals Base Sequence Chromosome Mapping Chromosomes, Human, Pair 8 Cloning, Molecular Gene Expression Glycoproteins/genetics,metabolism Humans In Situ Hybridization Mice Mice, Inbred C57BL Molecular Sequence Data Neuregulins Oligodeoxyribonucleotides/chemistry Oncogene Proteins, Viral/genetics RNA, Messenger/genetics Receptor, ErbB-2 Transcription, Genetic
Chemicals
Glycoproteins Neuregulins Oligodeoxyribonucleotides Oncogene Proteins, Viral RNA, Messenger Receptor, ErbB-2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Orr-Urtreger A
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Trakhtenbrot L
Ben-Levy R
Wen D
Rechavi G
Lonai P
Yarden Y
References (36)
36 references, click to expand
  1. New Giemsa method for the differential staining of sister chromatids.
    Nature. 1974 Sep 13;251(5471):156-8 PMID: 4138930
  2. Platelet-derived growth factor-A and its receptor are expressed in separate, but adjacent cell layers of the mouse embryo.
    Development. 1992 Aug;115(4):1045-58 PMID: 1451656
  3. Evidence of clonal attenuation, clonal succession, and clonal expansion in mass cultures of aging Werner's syndrome skin fibroblasts.
    Cytogenet Cell Genet. 1981;30(2):108-17 PMID: 7273855
  4. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  5. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen.
    Nature. 1984 Dec 6-12;312(5994):513-6 PMID: 6095109
  6. High-resolution chromosomal localization of the beta-gene of the human beta-globin gene complex by in situ hybridization.
    Cytogenet Cell Genet. 1985;39(4):269-74 PMID: 4053691
  7. p185, a product of the neu proto-oncogene, is a receptorlike protein associated with tyrosine kinase activity.
    Mol Cell Biol. 1986 May;6(5):1729-40 PMID: 2878363
  8. Activated neu oncogene sequences in primary tumors of the peripheral nervous system induced in rats by transplacental exposure to ethylnitrosourea.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6317-21 PMID: 3476947
  9. Stage- and tissue-specific expression of the neu oncogene in rat development.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8498-501 PMID: 3317411
  10. Genetic alterations during colorectal-tumor development.
    N Engl J Med. 1988 Sep 1;319(9):525-32 PMID: 2841597
  11. Clonally related cortical cells show several migration patterns.
    Science. 1988 Sep 9;241(4871):1342-5 PMID: 3137660
  12. Expression of the c-fms proto-oncogene and of the cytokine, CSF-1, during mouse embryogenesis.
    Dev Biol. 1989 May;133(1):284-94 PMID: 2523324
  13. A chimeric EGF-R-neu proto-oncogene allows EGF to regulate neu tyrosine kinase and cell transformation.
    EMBO J. 1989 Jan;8(1):159-66 PMID: 2565807
  14. HER2 cytoplasmic domain generates normal mitogenic and transforming signals in a chimeric receptor.
    EMBO J. 1989 Jan;8(1):167-73 PMID: 2565808
  15. Experimental approaches to hypothetical hormones: detection of a candidate ligand of the neu protooncogene.
    Proc Natl Acad Sci U S A. 1989 May;86(9):3179-83 PMID: 2470093
  16. Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer.
    Science. 1989 May 12;244(4905):707-12 PMID: 2470152
  17. Agonistic antibodies stimulate the kinase encoded by the neu protooncogene in living cells but the oncogenic mutant is constitutively active.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2569-73 PMID: 1969636
  18. Signal transduction by receptors with tyrosine kinase activity.
    Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
  19. Intermolecular association of the p185neu protein and EGF receptor modulates EGF receptor function.
    Cell. 1990 Jun 29;61(7):1339-47 PMID: 1973074
  20. Transforming growth factor-alpha. A model for membrane-anchored growth factors.
    J Biol Chem. 1990 Dec 15;265(35):21393-6 PMID: 2254298
  21. Heterodimerization of the erbB-1 and erbB-2 receptors in human breast carcinoma cells: a mechanism for receptor transregulation.
    Biochemistry. 1990 Dec 18;29(50):11024-8 PMID: 1980216
  22. The erbB-2 mitogenic signaling pathway: tyrosine phosphorylation of phospholipase C-gamma and GTPase-activating protein does not correlate with erbB-2 mitogenic potency.
    Mol Cell Biol. 1991 Apr;11(4):2040-8 PMID: 1672440
  23. Biochemical analysis of the ligand for the neu oncogenic receptor.
    Biochemistry. 1991 Apr 9;30(14):3543-50 PMID: 1672825
  24. Oncogenic forms of the neu/HER2 tyrosine kinase are permanently coupled to phospholipase C gamma.
    EMBO J. 1991 Aug;10(8):2077-86 PMID: 1676673
  25. Mechanistic aspects of the opposing effects of monoclonal antibodies to the ERBB2 receptor on tumor growth.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8691-5 PMID: 1717984
  26. Genetic linkage of Werner's syndrome to five markers on chromosome 8.
    Nature. 1992 Feb 20;355(6362):735-8 PMID: 1741060
  27. Isolation of the neu/HER-2 stimulatory ligand: a 44 kd glycoprotein that induces differentiation of mammary tumor cells.
    Cell. 1992 Apr 3;69(1):205-16 PMID: 1348215
  28. Expression of the neu proto-oncogene by Schwann cells during peripheral nerve development and Wallerian degeneration.
    J Neurosci Res. 1992 Apr;31(4):622-34 PMID: 1374476
  29. Neu differentiation factor: a transmembrane glycoprotein containing an EGF domain and an immunoglobulin homology unit.
    Cell. 1992 May 1;69(3):559-72 PMID: 1349853
  30. Identification of heregulin, a specific activator of p185erbB2.
    Science. 1992 May 22;256(5060):1205-10 PMID: 1350381
  31. Regulated coupling of the Neu receptor to phosphatidylinositol 3'-kinase and its release by oncogenic activation.
    J Biol Chem. 1992 Jun 15;267(17):12266-74 PMID: 1351056
  32. Angiogenesis during human extraembryonic development involves the spatiotemporal control of PDGF ligand and receptor gene expression.
    Development. 1991 Nov;113(3):749-54 PMID: 1668277
  33. Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists.
    J Biol Chem. 1992 Jul 25;267(21):15160-7 PMID: 1353079
  34. Quantitative acute leukemia cytogenetics.
    Genes Chromosomes Cancer. 1992 Jul;5(1):57-66 PMID: 1384663
  35. Deletions in the short arm of chromosome 8 are present in up to 90% of human colorectal cancer cell lines.
    Genes Chromosomes Cancer. 1992 Jul;5(1):91-5 PMID: 1384668
  36. High resolution R- and G-banding on the same preparation.
    Hum Genet. 1981;57(1):93-5 PMID: 7262875
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.5.1867
Published
1993-03-01
Pages
1867-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45981
Subset
IM
Grants
NCI NIH HHS · CA 51712 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.5.1867 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2