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PMID: 808223 Published · ppublish English Journal Article

The topography of lactose synthesis.

The Biochemical journal ·Vol. 148 ·No. 1 ·1975-04-00 ·Pages 77-84

Kuhn NJ, White A

Abstract

1. At short incubation times, and under suitable osmotic conditions, the lactose synthesized by Golgi-derived vesicles of rat mammary gland is 85-90% particulate. Evidence is presented for its occlusion within the lumen of the vesicles. 2. Ovalbumin is used as a bulky active-site inhibitor to show that the active site of lactose synthase lies on the inner face of the Golgi membrane. 3. Phlorrhizin and phloretin inhibit lactose synthesis by such vesicles, indicating the presence of a glucose-transport system. 4. The relationship of this topography to the synthesis of N-acetylneuraminyl-lactose and to the secretion of milk sugars is discussed.

MeSH Terms
Animals Binding Sites Biological Transport Female Glucose/metabolism Golgi Apparatus/drug effects,metabolism Lactose/biosynthesis Lactose Synthase/metabolism Mammary Glands, Animal/metabolism Ovalbumin Phloretin/pharmacology Phlorhizin/pharmacology Pregnancy Rats Subcellular Fractions
Chemicals
Ovalbumin Phlorhizin Lactose Synthase Glucose Lactose Phloretin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuhn N J
White A
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23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1480077
Published
1975-04-00
Pages
77-84
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165508
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1480077 PMC Full Text View journal details
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