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PMID: 4362333 Published · ppublish English Journal Article

Progesterone and the metabolic control of the lactose biosynthetic pathway during lactogenesis in the rat.

The Biochemical journal ·Vol. 136 ·No. 4 ·1973-12-00 ·Pages 1105-16

Murphy G, Ariyanayagam AD, Kuhn NJ

Abstract

1. Lactogenesis was initiated in pregnant rats by ovariectomy, thereby causing progesterone withdrawal, after which the mammary tissue was analysed for contents of enzymes and metabolites concerned with the biosynthesis of lactose. 2. Lactose synthesis increased about 126-fold with little or no accompanying change in the contents of most metabolic intermediates or in the adenine nucleotide energy charge. 3. Comparison of mass-action ratios with equilibrium constants showed that phosphoglucomutase (EC 2.7.5.1), UDP-glucose pyrophosphorylase (EC 2.7.7.9) and UDP-glucose epimerase (EC 5.1.3.2.) catalysed reactions close to equilibrium. Nucleoside diphosphokinase (EC 2.7.4.6.) activity was very high and probably equilibrates the UTP-UDP and ATP-ADP couples. Lactose synthetase and hexokinase (EC 2.7.1.1) appeared to catalyse rate-limiting reactions. 4. Large increases were seen of UDP-glucose pyrophosphorylase (5-fold), lactose synthetase A protein (3.8-fold) and alpha-lactalbumin (28-fold), but not of hexokinase, phosphoglucomutase, UDP-glucose epimerase, nucleoside diphosphokinase or glucose 6-phosphate dehydrogenase (EC 1.1.1.49) activities. 5. It appeared that the increased lactose synthesis was largely accounted for by the increased lactose synthetase A protein activity and alpha-lactalbumin.

MeSH Terms
Adenine Nucleotides/metabolism Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Animals Carbohydrate Epimerases/metabolism Caseins/metabolism Castration Female Glucosephosphate Dehydrogenase/metabolism Glucosephosphates Hexokinase Kinetics Lactalbumin/metabolism Lactates/metabolism Lactation Lactose/biosynthesis,urine Mammary Glands, Animal/metabolism Nucleotides Nucleotidyltransferases/metabolism Phosphoglucomutase/metabolism Phosphotransferases/metabolism Pregnancy Progesterone/physiology Pyruvates/metabolism Rats Uracil Nucleotides/metabolism Uridine Diphosphate Sugars
Chemicals
Adenine Nucleotides Caseins Glucosephosphates Lactates Nucleotides Pyruvates Uracil Nucleotides Uridine Diphosphate Sugars Progesterone Adenosine Diphosphate Adenosine Triphosphate Lactalbumin Glucosephosphate Dehydrogenase Phosphotransferases Hexokinase Nucleotidyltransferases Carbohydrate Epimerases Phosphoglucomutase Lactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murphy G
Ariyanayagam A D
Kuhn N J
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50 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1361105
Published
1973-12-00
Pages
1105-16
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166063
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1361105 PMC Full Text View journal details
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