The effects of starvation and alloxan-diabetes on the contents of citrate and other metabolic intermediates in rat liver.

The Biochemical journal ·Vol. 107 ·No. 3 ·1968-04-00 ·Pages 411-5

Start C, Newsholme EA

Abstract

1. The content of citrate in ;freeze-clamped' livers from starved and alloxan-diabetic rats was measured by using the specific citrate assay method of Gruber & Moellering (1966). 2. The content of citrate fell progressively during a period of 48hr. starvation to reach a plateau value that is 50% of the value for livers from fed rats. Some possible explanations for the conflicting reports of changes in hepatic citrate content during starvation are discussed. 3. The hepatic contents of ATP, pyruvate, lactate, glycogen and the hexose phosphates were decreased during starvation, whereas those of acetyl-CoA and AMP were increased. 4. Acute alloxan-diabetes produced similar changes in the contents of these metabolic intermediates. 5. The effects of starvation and diabetes on the citrate and acetyl-CoA contents are discussed in relation to control of gluconeogenesis, fatty acid synthesis and the activity of citrate synthase.

MeSH Terms

Adenine Nucleotides/metabolism Adenosine Triphosphate/metabolism Animals Carbon Isotopes Citrates/metabolism Coenzyme A/metabolism Diabetes Mellitus, Experimental/metabolism Freezing Hexosephosphates/metabolism Lactates/metabolism Liver/metabolism Liver Glycogen/metabolism Male Pyruvates/metabolism Rats Spectrophotometry Starvation/metabolism Time Factors Tritium

Chemicals

Adenine Nucleotides Carbon Isotopes Citrates Hexosephosphates Lactates Liver Glycogen Pyruvates Tritium Adenosine Triphosphate Coenzyme A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Start C

Newsholme E A

References (25)

25 references, click to expand

Variations in tissue contents of coenzyme A thio esters and possible metabolic implications.
Biochem J. 1964 Dec;93(3):550-7 PMID: 5839190
Regulation of glucose uptake by muscle. 7. Effects of fatty acids, ketone bodies and pyruvate, and of alloxan-diabetes, starvation, hypophysectomy and adrenalectomy, on the concentrations of hexose phosphates, nucleotides and inorganic phosphate in perfused rat heart.
Biochem J. 1964 Dec;93(3):641-51 PMID: 4220951
Regulation of glucose uptake by muscles. 10. Effects of alloxan-diabetes, starvation, hypophysectomy and adrenalectomy, and of fatty acids, ketone bodies and pyruvate, on the glycerol output and concentrations of free fatty acids, long-chain fatty acyl-coenzyme A, glycerol phosphate and citrate-cycle intermediates in rat heart and diaphragm muscles.
Biochem J. 1964 Dec;93(3):678-87 PMID: 5839199
ATP controlled acetoacetate and citrate synthesis by rat liver mitochondria oxidising palmitoyl-carnitine, and the inhibition of citrate synthase by ATP.
Biochem Biophys Res Commun. 1966 Jan 4;22(1):89-93 PMID: 5937344
The effects of adenine nucleotides on carbohydrate metabolism in pigeon-liver homogenates.
Biochem J. 1966 Mar;98(3):720-35 PMID: 4287841
Effects of changes in brain metabolism on the levels of citric acid cycle intermediates.
J Biol Chem. 1966 Sep 10;241(17):3997-4003 PMID: 5922095
The cellular control of fatty acid synthesis at the enzymatic level.
Biochem Soc Symp. 1963;24:43-56 PMID: 5335321
Citrate metabolism in diabetes. II. Tissue citrate content, citrate synthase and oxidation in alloxan-diabetic rat.
Metabolism. 1967 Mar;16(3):285-93 PMID: 6020737
[On inhibition of acetyl-CoA-carboxylase by fatty acid-coenzyme A compounds].
Biochem Z. 1965 Dec 1;343(3):243-57 PMID: 5875764
Studies on the ketogenic effect of glucagon in intact rat liver.
Biochem Biophys Res Commun. 1966 Aug 12;24(3):437-42 PMID: 5967108
Measurement of concentrations of metabolites in adipose tissue and effects of insulin, alloxan-diabetes and adrenaline.
Biochem J. 1966 Aug;100(2):407-19 PMID: 5968539
Citrate content of liver and kidney of rat in various metabolic states and in fluoroacetate poisoning.
Biochem J. 1967 May;103(2):342-8 PMID: 6032977
Control of glycolysis and gluconeogenesis in rat kidney cortex slices.
Biochem J. 1967 Jul;104(1):300-5 PMID: 4292000
Control of glycolysis in cerebral cortex slices.
Biochem J. 1967 Aug;104(2):524-33 PMID: 4227784
Effects of starvation and alloxan diabetes on the content of citrate in the livers of the rat.
Biochem J. 1967 Sep;104(3):46P-47P PMID: 6049879
Control of glycolysis and gluconeogenesis in liver and kidney cortex.
Vitam Horm. 1967;25:1-87 PMID: 4868322
The purification of aconitase.
Biochem J. 1954 Jan;56(1):99-105 PMID: 13126098
Some observations concerning hepatic glucose 6-phosphate content in normal and diabetic rats.
J Biol Chem. 1959 Jun;234(6):1342-6 PMID: 13654375
Kinetic studies of liver alcohol dehydrogenase.
Biochem J. 1962 Aug;84:244-54 PMID: 13883267
Enzyme patterns in rat liver and Morris hepatoma 5123 during metabolic transitions.
Cold Spring Harb Symp Quant Biol. 1961;26:355-62 PMID: 14488303
The purification of citrate-condensing enzyme.
J Biol Chem. 1961 Oct;236:2557-9 PMID: 13915993
The supply of precursors for the synthesis of fatty acids.
J Biol Chem. 1962 Dec;237:3640-8 PMID: 13990010
Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices.
Biochem J. 1963 Jan;86:22-7 PMID: 14035641
REGULATION OF PHOSPHOFRUCTOKINASE ACTIVITY BY CITRATE IN NORMAL AND DIABETIC MUSCLE.
Biochem Biophys Res Commun. 1963 Aug 1;12:268-73 PMID: 14070328
PROPERTIES OF PHOSPHOFRUCTOKINASE FROM RAT LIVER AND THEIR RELATION TO THE CONTROL OF GLYCOLYSIS AND GLUCONEOGENESIS.
Biochem J. 1965 Jun;95:868-75 PMID: 14342527

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1070411

Published

1968-04-00

Pages

411-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1198678

Subset

External Links

PubMed source DOI: 10.1042/bj1070411 PMC Full Text View journal details

Analysis Services

The effects of starvation and alloxan-diabetes on the contents of citrate and other metabolic intermediates in rat liver.

Contact

WeChat Official Account