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PMID: 6032977 Published · ppublish English Journal Article

Citrate content of liver and kidney of rat in various metabolic states and in fluoroacetate poisoning.

The Biochemical journal ·Vol. 103 ·No. 2 ·1967-05-00 ·Pages 342-8

Spencer AF, Lowenstein JM

Abstract

The citrate content of rat liver changes little when normal rats are starved, when starved rats are re-fed with various diets and when normal animals are made diabetic with alloxan. The citrate content of rat kidney changes little on starvation, but it doubles on induction of diabetes. Fluoroacetate poisoning has relatively little effect on the citrate content of liver under a variety of conditions except that normal female rats show a 2.4-fold increase. Fluoroacetate poisoning leads to increases in the citrate content of kidney under all conditions. The relevance of these observations to the regulation of fatty acid synthesis is discussed. The acetic anhydride-pyridine method and the pentabromoacetone method for the estimation of citrate are compared.

MeSH Terms
Animals Citrates/analysis,metabolism Diabetes Mellitus, Experimental/metabolism Diet Fatty Acids/biosynthesis Female Fluoroacetates/poisoning Injections, Intraperitoneal Kidney/metabolism Liver/metabolism Male Rats Sex Spectrophotometry Starvation/metabolism Tissue Extracts
Chemicals
Citrates Fatty Acids Fluoroacetates Tissue Extracts
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spencer A F
Lowenstein J M
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35 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030342
Published
1967-05-00
Pages
342-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270414
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030342 PMC Full Text View journal details
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