Home LiteratureArticle Details
PMID: 807561 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.

Journal of bacteriology ·Vol. 122 ·No. 3 ·1975-06-00 ·Pages 987-93

Bettinger GE, Young FE

Abstract

The transformation of Bacillus subtilis by homologous deoxyribonucleic acid (DNA) made available by gently lysing a stable L-form or protoplast suspension was 3 to 10-fold more efficient than DNA isolated by conventional procedures. This increased transformation was not influenced by digestion with pronase, trypsin, or ribonuclease. Preincubation of isolated DNA with L-form lysates did not increase the transformation efficiency above that achieved with untreated, isolated DNA. In addition to displaying a higher efficiency of transformation, the DNA found in these gently prepared lysates was also able to co-transform heretofore unlinked markers at frequencies in excess of those found by congression. Comparison of the frequency of multiple marker transformations to single marker events as a function of DNA dilution conclusively proves that these markers originated from the same continuous strand of DNA.

MeSH Terms
Bacillus subtilis/metabolism Bacteriolysis DNA, Bacterial/metabolism Deoxyribonucleases/metabolism Genes L Forms Mutation Pronase/metabolism Protoplasts Ribonucleases/metabolism Thymidine/metabolism Transformation, Genetic Tritium Trypsin/metabolism
Chemicals
DNA, Bacterial Tritium Deoxyribonucleases Ribonucleases Trypsin Pronase Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bettinger G E
Young F E
References (27)
27 references, click to expand
  1. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  2. UNSTABLE LINKAGE BETWEEN GENETIC MARKERS IN TRANSFORMATION.
    J Bacteriol. 1965 May;89:1314-21 PMID: 14293004
  3. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  4. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.
    J Bacteriol. 1965 Mar;89:782-90 PMID: 14273661
  5. CONFIGURATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID DURING ENTRY INTO BACILLUS SUBTILIS.
    J Bacteriol. 1965 Feb;89:288-93 PMID: 14255692
  6. Studies on transformations of Hemophilus influenzae. IV. Linked and unlinked transformations.
    J Gen Physiol. 1961 Nov;45:205-28 PMID: 13900279
  7. Linkage of genetic units of Bacillus subtilis in DNA transformation.
    Proc Natl Acad Sci U S A. 1961 Jan 15;47:52-5 PMID: 13728419
  8. Conditions affecting the isolation from transformed cells of Bacillus subtilis of high-molecular-weight single-stranded deoxyribonucleic acid of donor origin.
    J Bacteriol. 1973 Oct;116(1):146-53 PMID: 4200839
  9. Structure and replication of chromosomes in competent cells of Bacillus subtilis.
    J Bacteriol. 1972 Mar;109(3):1075-84 PMID: 4110922
  10. Transformation of Bacillus subtilis using gently lysed L-forms; a new mapping technique.
    Biochem Biophys Res Commun. 1973 Dec 19;55(4):1105-11 PMID: 4203903
  11. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.
    J Bacteriol. 1972 Apr;110(1):281-90 PMID: 4622900
  12. Deoxyribonucleic acid-membrane complexes in the Bacillus subtilis transformation system.
    J Bacteriol. 1973 May;114(2):711-22 PMID: 4196254
  13. Transformation in Bacillus subtilis. Fate of newly introduced transforming DNA.
    Mol Gen Genet. 1973;123(2):185-98 PMID: 4199220
  14. Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: size and distribution of the integrated donor segments.
    J Bacteriol. 1972 Aug;111(2):488-94 PMID: 4626501
  15. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.
    J Mol Biol. 1972 Feb 28;64(1):9-29 PMID: 4622632
  16. Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA.
    J Mol Biol. 1972 Feb 28;64(1):31-46 PMID: 4622631
  17. Congression of unlinked markers and genetic mapping in the transformation of Bacillus subtilis 168.
    Genetics. 1973 Jan;73(1):13-21 PMID: 4631599
  18. The influence of temperate bacteriophage phi105 on transformation and transfection in Bacillus subtilis.
    Biochem Biophys Res Commun. 1972 Apr 28;47(2):365-71 PMID: 4196974
  19. Transfecting deoxyribonucleic acid of Bacillus bacteriophage phi 29 that is protease sensitive.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1555-9 PMID: 4504368
  20. Isolation of L-forms of Bacillus subtilis which grow in liquid medium.
    J Bacteriol. 1970 Jun;102(3):867-70 PMID: 4988045
  21. Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.
    J Bacteriol. 1970 Nov;104(2):839-50 PMID: 4992373
  22. Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.
    Biochem Biophys Res Commun. 1969 May 8;35(3):354-62 PMID: 4977972
  23. A rapid method for synchronization of Staphylococcus aureus and Bacillus subtilis.
    Biochem Biophys Res Commun. 1971 Sep;44(5):1125-30 PMID: 5003662
  24. Mechanism of transformation in B. subtilis.
    Mol Gen Genet. 1971;113(4):331-44 PMID: 5004186
  25. Further evidence concerning the configuration of transforming deoxyribonucleic acid during entry into Bacillus subtilis.
    J Bacteriol. 1966 Feb;91(2):702-8 PMID: 4956757
  26. Apparent dependence of transformation on the stage of deoxyribonucleic acid replication of recipient cells.
    Bacteriol Rev. 1968 Dec;32(4 Pt 1):291-6 PMID: 4974083
  27. Characteristics of penicillinase release by washed cells of Bacillus licheniformis.
    J Bacteriol. 1968 Oct;96(4):1231-9 PMID: 5685998
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.122.3.987-993.1975
Published
1975-06-00
Pages
987-93
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246150
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.122.3.987-993.1975 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2