Deoxyribonucleic acid-membrane complexes in the Bacillus subtilis transformation system.

Journal of bacteriology ·Vol. 114 ·No. 2 ·1973-05-00 ·Pages 711-22

Dooley DC, Nester EW

Abstract

The fate of transforming deoxyribonucleic acid (DNA) in Bacillus subtilis was studied by isolating the DNA-membrane complex on Renografin gradients. Soon after uptake, transforming DNA binds to the cell membrane and displays a greater resistance to shear than the recipient genome-membrane complex. Ten minutes after uptake, a portion of the donor DNA is released from the membrane. Most of the released donor radioactivity represents unintegrated, biologically inactive DNA. Recombinant or integrated DNA is enriched 1.5- to 1.7-fold in the membrane. This enrichment last at least 30 min after termination of DNA uptake, and probably much longer. The data suggest that transforming DNA may be integrated into the recipient genome on, or close to, the cell membrane.

MeSH Terms

Bacillus subtilis/growth & development Binding Sites Carbon Isotopes/metabolism Cell Membrane/metabolism Centrifugation, Density Gradient DNA, Bacterial/isolation & purification,metabolism Densitometry Diatrizoate Leucine/metabolism Recombination, Genetic Stereoisomerism Thymidine/metabolism Thymine Transformation, Genetic Tritium

Chemicals

Carbon Isotopes DNA, Bacterial Tritium Diatrizoate Leucine Thymine Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dooley D C

Nester E W

References (24)

24 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.114.2.711-722.1973

Published

1973-05-00

Pages

711-22

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251831

Subset

External Links

PubMed source DOI: 10.1128/jb.114.2.711-722.1973 PMC Full Text View journal details

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Deoxyribonucleic acid-membrane complexes in the Bacillus subtilis transformation system.

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