Fate of transforming deoxyribonucleate in Bacillus subtilis.

Journal of bacteriology ·Vol. 108 ·No. 2 ·1971-11-00 ·Pages 680-9

Piechowska M, Fox MS

Abstract

The majority of donor deoxyribonucleate (DNA) at early stages after uptake was found in a complex with a cell component which changes its buoyant behavior on equilibrium density gradients. Analysis of the recipient cell lysates, after treatment to dissociate the complex, showed about two-thirds of the donor molecules in denatured form and the rest associated with recipient DNA. Incubation of cells after DNA uptake leads to the disappearance of denatured donor DNA and to the increase of donor label associated with recipient DNA. Some characteristics of a component from intact cells or spheroplasts with affinity for denatured Bacillus subtilis DNA are described.

MeSH Terms

Bacillus subtilis/analysis,metabolism Carbon Isotopes Centrifugation, Density Gradient Cesium Chlorides DNA, Bacterial/analysis,metabolism Hot Temperature Hydrogen-Ion Concentration Lipase Muramidase Nucleic Acid Denaturation Peptide Hydrolases Salmonella typhimurium Sucrose Thymine/metabolism Transformation, Genetic Tritium

Chemicals

Carbon Isotopes Chlorides DNA, Bacterial Tritium Cesium Sucrose Lipase Muramidase Peptide Hydrolases Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Piechowska M

Fox M S

References (16)

16 references, click to expand

Integration efficiency and genetic recombination in pneumococcal transformation.
Genetics. 1966 Jan;53(1):207-35 PMID: 4379022
On the mechanism of integration of transforming deoxyribonucleate.
J Gen Physiol. 1966 Jul;49(6):183-96 PMID: 4381781
Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules.
J Mol Biol. 1966 Nov 14;21(2):247-53 PMID: 4291402
Characterization of the plasma membrane of Mycoplasma laidlawii. I. Sodium dodecyl sulfate solubilization.
Biochim Biophys Acta. 1967 Jul 3;135(3):381-90 PMID: 6048810
Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.
J Bacteriol. 1968 Mar;95(3):867-75 PMID: 4966830
The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis.
Biochem J. 1969 Jun;113(1):167-74 PMID: 4979894
T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.
Nature. 1970 Sep 26;227(5265):1313-8 PMID: 5455134
Synthesis and maturation of phage P22 DNA. I. Identification of intermediates.
J Mol Biol. 1968 Jun 28;34(3):621-41 PMID: 4938561
Molecular fate of DNA in genetic transformation of Pneumococcus.
J Mol Biol. 1962 Jul;5:119-31 PMID: 14461406
The bouyant behavior of viral and bacterial DNA in alkaline CsCl.
Proc Natl Acad Sci U S A. 1963 Jan 15;49:12-7 PMID: 13997384
ON THE MECHANISM OF GENETIC RECOMBINATION IN TRANSFORMING BACILLUS SUBTILIS.
J Mol Biol. 1964 Jul;9:236-45 PMID: 14200386
ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.
Proc Natl Acad Sci U S A. 1964 Aug;52:412-9 PMID: 14206608
BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION.
Genetics. 1964 Oct;50:717-38 PMID: 14221877
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.
J Bacteriol. 1965 May;89:1250-5 PMID: 14292994
PROPERTIES OF NEWLY INTRODUCED TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.
J Bacteriol. 1965 Aug;90:343-6 PMID: 14329445

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.2.680-689.1971

Published

1971-11-00

Pages

680-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247126

Subset

External Links

PubMed source DOI: 10.1128/jb.108.2.680-689.1971 PMC Full Text View journal details

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Fate of transforming deoxyribonucleate in Bacillus subtilis.

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