Improved culture flask for obligate anaerobes.

Applied microbiology ·Vol. 29 ·No. 5 ·1975-05-00 ·Pages 710-1

Daniels L, Zeikus JG

Abstract

An improved flask system for the growth of extremely oxygen-sensitive bacteria in liquid culture is described. The improvement described utilizes an all-glass, neoprene-stoppered flask designed for growth of 50- to 1,000-ml cultures of bacteria with continuous gassing.

MeSH Terms

Anaerobiosis Bacteria/growth & development Bacteriological Techniques/instrumentation Clostridium/growth & development Evaluation Studies as Topic Glass Micrococcaceae/growth & development Neoprene

Chemicals

Neoprene

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Daniels L

Zeikus J G

References (6)

6 references, click to expand

Gas-tight flask for the concurrent measurement of gas metabolism and growth in methane-oxidizing bacteria.
Appl Microbiol. 1974 Sep;28(3):507-9 PMID: 4609210
A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.
Appl Microbiol. 1974 May;27(5):985-7 PMID: 4598231
Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile.
J Bacteriol. 1972 Feb;109(2):707-15 PMID: 4550816
Apparatus for metabolic studies with anaerobes.
Appl Microbiol. 1971 Mar;21(3):555-6 PMID: 4928609
A simple culture vessel for turbidimetric studies of anaerobie growth of microbes.
Experientia. 1968 Oct 15;24(10):1076-7 PMID: 5711888
Hydrogen-oxidizing methane bacteria. I. Cultivation and methanogenesis.
J Bacteriol. 1968 Mar;95(3):1118-23 PMID: 5651323

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.29.5.710-711.1975

Published

1975-05-00

Pages

710-1

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC187062

Subset

External Links

PubMed source DOI: 10.1128/am.29.5.710-711.1975 PMC Full Text

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