A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.

Applied microbiology ·Vol. 27 ·No. 5 ·1974-05-00 ·Pages 985-7

Miller TL, Wolin MJ

Abstract

The modification described utilizes a serum bottle closed with a butyl rubber stopper with a crimped metal seal for the growth of obligately anaerobic bacteria.

MeSH Terms

Anaerobiosis Bacteria/growth & development Bacteriological Techniques/instrumentation Bacteroides/growth & development Methods

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Miller T L

Wolin M J

References (3)

3 references, click to expand

Use of syringe methods for anaerobiosis.
Am J Clin Nutr. 1972 Dec;25(12):1318-23 PMID: 4565348
Commentary on the Hungate technique for culture of anaerobic bacteria.
Am J Clin Nutr. 1972 Dec;25(12):1324-8 PMID: 4565349
Formation of hydrogen and formate by Ruminococcus albus.
J Bacteriol. 1973 Nov;116(2):836-46 PMID: 4745433

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.27.5.985-987.1974

Published

1974-05-00

Pages

985-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380188

Subset

External Links

PubMed source DOI: 10.1128/am.27.5.985-987.1974 PMC Full Text

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A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.

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