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PMID: 806521 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunogenicity of ribosomal vaccines isolated from group A, type 14 Streptococcus pyogenes.

Infection and immunity ·Vol. 11 ·No. 6 ·1975-06-00 ·Pages 1195-202

Schalla WO, Johnson W

Abstract

A ribosomal preparation isolated from group A, type 14 Streptococcus pyogenes protected mice against a challenge of 1,000 mean lethal doses. Optimal immunization consisted of subcutaneous administration of 326 mug of protein with Freund incomplete adjuvant. Animals immunized with higher or lower antigen concentrations showed decreased survival. Freund incomplete adjuvant was necessary to insure optimum immunization. Immunochemical studies showed no M protein or C carbohydrate present in the ribosomal preparation. Mice optimally immunized with the type 14 ribosomal preparation were protected against heterologous challenge with M types 2, 5, 8, and 12. Protein-free ribonucleic acid extracts isolated from the type 14 ribosomal preparation did not protect mice against homologous challenge. But ribonucleic acid-free protein extracts showed 100% protection in mice after homologous challenge.

MeSH Terms
Animals Bacterial Vaccines/isolation & purification Blood/microbiology Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Immunization Immunodiffusion Mice RNA, Ribosomal/isolation & purification Ribosomal Proteins/isolation & purification Spleen/microbiology Streptococcus pyogenes/immunology Ultracentrifugation
Chemicals
Bacterial Vaccines RNA, Ribosomal Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schalla W O
Johnson W
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25 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.6.1195-1202.1975
Published
1975-06-00
Pages
1195-202
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415199
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.6.1195-1202.1975 PMC Full Text View journal details
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