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PMID: 8062832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A transcriptional silencer controls the developmental expression of the CD4 gene.

The EMBO journal ·Vol. 13 ·No. 15 ·1994-08-01 ·Pages 3570-9

Siu G, Wurster AL, Duncan DD, Soliman TM, Hedrick SM

Abstract

The appropriate expression of the CD4 glycoprotein is required for T-cell function and development. Here we define the transcriptional control elements in the CD4 locus that convey CD(4+)-specific expression of a marker gene in transgenic mice. Using nuclear run-on experiments, we have determined that the major mechanism for CD4 expression control during development is transcriptional. We have identified a developmental stage- and tissue-specific negative regulatory element in the first intron of the murine CD4 gene that has the characteristics of a transcriptional silencer. The CD4 silencer functions to inhibit marker gene expression at two different stages of T-cell development, as well as in non-T hematopoietic cells, and thus is the critical controlling element responsible for T-cell-specific, as well as developmental- and subclass-specific, expression.

MeSH Terms
Animals CD4 Antigens/genetics CD8 Antigens/genetics Cells, Cultured Enhancer Elements, Genetic Gene Expression Regulation/physiology Genetic Markers HLA-B7 Antigen/genetics Humans Mice Mice, Transgenic Models, Genetic Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid/physiology Spleen/cytology T-Lymphocyte Subsets/physiology Thymus Gland/cytology Transcription, Genetic/physiology
Chemicals
CD4 Antigens CD8 Antigens Genetic Markers HLA-B7 Antigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Siu G
Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY 10032.
Wurster A L
Duncan D D
Soliman T M
Hedrick S M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-08-01
Pages
3570-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395261
Subset
IM
Grants
NIAID NIH HHS · AI29990 · United States
External Links
PubMed source PMC Full Text View journal details
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