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PMID: 8056305 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of autoregulation at the level of pre-mRNA splicing of the suppressor-of-white-apricot gene in Drosophila.

Genetics ·Vol. 137 ·No. 1 ·1994-05-00 ·Pages 139-50

Zachar Z, Chou TB, Kramer J, Mims IP, Bingham PM

Abstract

The Drosophila suppressor-of-white-apricot [su(wa)] protein regulates/modulates at least two somatic RNA processing events. It is a potent regulator of its own expression. We report here new studies of this autoregulatory circuit. Among other things, our studies show the following. First, new evidence that su(wa) expression is autoregulated at the level of pre-mRNA splicing is reported. su(wa) protein represses accumulation of the fully spliced su(wa) mRNA encoding it and promotes accumulation of high levels of incompletely spliced su(wa) pre-mRNA. Second, the fully spliced su(wa) mRNA is sufficient for all known su(wa) genetic functions indicating that it encodes the sole su(wa) protein. Third, the incompletely spliced su(wa) pre-mRNAs resulting from autoregulation are not translated (probably as a result of nuclear retention) and apparently represent nonfunctional by-products. Fourth, the special circumstances of su(wa) expression during oogenesis allows maternal deposition exclusively of fully spliced su(wa) mRNA. Fifth, su(wa) protein immunolocalizes to nuclei consistent with its being a direct regulator of pre-mRNA processing. We discuss the implications of our results for mechanisms of splicing regulation and for developmental control of su(wa) expression.

MeSH Terms
Animals Cloning, Molecular Drosophila/genetics Drosophila Proteins Gene Expression Regulation Genes, Reporter Introns Nuclear Proteins/genetics,metabolism Protein Biosynthesis Proteins/genetics,metabolism RNA Splicing/genetics Transcription, Genetic
Chemicals
Drosophila Proteins Nuclear Proteins Proteins su(wa) protein, Drosophila
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zachar Z
Department of Biochemistry and Cell Biology, University of New York, Stony Brook 11794-5215.
Chou T B
Kramer J
Mims I P
Bingham P M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/137.1.139
Published
1994-05-00
Pages
139-50
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205931
Subset
IM
Grants
NIGMS NIH HHS · GM32003 · United States
External Links
PubMed source DOI: 10.1093/genetics/137.1.139 PMC Full Text View journal details
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