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PMID: 8049377 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone.

Plant molecular biology ·Vol. 25 ·No. 3 ·1994-06-00 ·Pages 545-50

Vauterin M, Jacobs M

Abstract

A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the poplar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Arabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.

Related Genes
dapA
MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Cloning, Molecular DNA, Complementary/genetics Genes, Plant/genetics Genomic Library Hydro-Lyases/genetics Molecular Sequence Data Plants/enzymology,genetics Polymerase Chain Reaction Sequence Homology, Amino Acid Trees/enzymology,genetics
Chemicals
DNA, Complementary Hydro-Lyases 4-hydroxy-tetrahydrodipicolinate synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vauterin M
Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, Belgium.
Jacobs M
References (18)
18 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00043882
Published
1994-06-00
Pages
545-50
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X72743, X72971
External Links
PubMed source DOI: 10.1007/BF00043882 View journal details
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