Abstract
Heat shock gene expression is induced by a variety of environmental stresses, including the presence of many chemicals. To address the utility of this response for pollutant detection, two Escherichia coli heat shock promoters, dnaK and grpE, were fused to the lux genes of Vibrio fischeri. Metals, solvents, crop protection chemicals, and other organic molecules rapidly induced light production from E. coli strains containing these plasmid-borne fusions. Introduction of an outer membrane mutation, tolC, enhanced detection of a hydrophobic molecule, pentachlorophenol. The maximal response to pentachlorophenol in the tolC+ strain was at 38 ppm, while the maximal response in an otherwise isogenic tolC mutant was at 1.2 ppm. Stress responses were observed in both batch and chemostat cultures. It is suggested that biosensors constructed in this manner may have potential for environmental monitoring.
MeSH Terms
Bacterial Outer Membrane Proteins/genetics
Bacterial Proteins/genetics
Base Sequence
Cloning, Molecular
DNA, Bacterial
Environmental Monitoring
Escherichia coli/genetics
Escherichia coli Proteins
HSP70 Heat-Shock Proteins
Heat-Shock Proteins/genetics
Light
Luminescence
Membrane Transport Proteins
Molecular Sequence Data
Mutation
Vibrio/genetics
Water Pollutants, Chemical/analysis
Chemicals
Bacterial Outer Membrane Proteins
Bacterial Proteins
DNA, Bacterial
Escherichia coli Proteins
GrpE protein, Bacteria
GrpE protein, E coli
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Membrane Transport Proteins
Water Pollutants, Chemical
tolC protein, E coli
dnaK protein, E coli
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Van Dyk T K
DuPont Company, Wilmington, Delaware 19880.
Majarian W R
Konstantinov K B
Young R M
Dhurjati P S
LaRossa R A
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