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PMID: 8005674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fimbria-mediated adherence of Candida albicans to glycosphingolipid receptors on human buccal epithelial cells.

Infection and immunity ·Vol. 62 ·No. 7 ·1994-07-00 ·Pages 2843-8

Yu L, Lee KK, Sheth HB, Lane-Bell P, Srivastava G, Hindsgaul O, Paranchych W, Hodges RS, Irvin RT

Abstract

Candida albicans is an opportunist fungal pathogen that has the ability to adhere to host cell surface receptors via a number of adhesins. Yu et al. (L. Yu, K. K. Lee, K. Ens, P. C. Doig, M. R. Carpenter, W. Staddon, R. S. Hodges, W. Paranchych, and R. T. Irvin, Infect. Immun. 62:2834-2842, 1994) described the purification and initial characterization of a fimbrial adhesin from C. albicans. In this paper, we show that C. albicans fimbriae also bind to asialo-GM1 [gangliotetraosylceramide: beta Gal(1-3)beta GalNAc(1-4) beta Gal(1-4)beta Glc(1-1)Cer] immobilized on microtiter plates in a saturable and concentration-dependent manner. C. albicans fimbrial binding to exfoliated human buccal epithelial cells (BECs) was inhibited by asialo-GM1 in in vitro binding assays. The fimbriae interact with the glycosphingolipid receptors via the carbohydrate portion of the receptors, since fimbriae were observed to bind to synthetic beta GalNAc(1-4)beta Gal-protein conjugates and the disaccharide was able to inhibit binding of fimbriae to BECs in in vitro binding assays. We conclude from these results that the C. albicans yeast form expresses a fimbrial adhesin that binds to glycosphingolipids displayed on the surface of human BECs.

MeSH Terms
Candida albicans/physiology Cell Adhesion/physiology Cell Adhesion Molecules/metabolism Epithelial Cells Epithelium/metabolism Fungal Proteins/metabolism Glycoproteins/metabolism Glycosphingolipids/metabolism Humans Male Mouth/cytology,metabolism Organelles/physiology Protein Binding Receptors, Cell Surface/metabolism
Chemicals
Cell Adhesion Molecules Fungal Proteins Glycoproteins Glycosphingolipids Receptors, Cell Surface
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Yu L
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Lee K K
Sheth H B
Lane-Bell P
Srivastava G
Hindsgaul O
Paranchych W
Hodges R S
Irvin R T
References (52)
52 references, click to expand
  1. Adherence of Candida albicans to human vaginal and buccal epithelial cells.
    J Infect Dis. 1981 Jan;143(1):76-82 PMID: 7012245
  2. Relationship between germination of Candida albicans and increased adherence to human buccal epithelial cells.
    Infect Immun. 1980 May;28(2):464-8 PMID: 6995309
  3. Modulation of Candida albicans attachment to human epithelial cells by bacteria and carbohydrates.
    Infect Immun. 1983 Mar;39(3):1354-60 PMID: 6132878
  4. Variability of the adherence of Candida albicans strains to human buccal epithelial cells: inconsistency of differences between strains related to virulence.
    Sabouraudia. 1983 Jun;21(2):93-8 PMID: 6348979
  5. Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro.
    Infect Immun. 1983 Sep;41(3):1024-30 PMID: 6350176
  6. Adherence and penetration of vascular endothelium by Candida yeasts.
    Infect Immun. 1983 Oct;42(1):374-84 PMID: 6352500
  7. Adherence of Candida species to human epidermal corneocytes and buccal mucosal cells: correlation with cutaneous pathogenicity.
    J Invest Dermatol. 1984 Jul;83(1):37-41 PMID: 6429252
  8. Inhibition by sugars of Candida albicans adherence to human buccal mucosal cells and corneocytes in vitro.
    Infect Immun. 1984 Dec;46(3):831-4 PMID: 6389365
  9. Extracellular polymer of Candida albicans: isolation, analysis and role in adhesion.
    J Gen Microbiol. 1985 Mar;131(3):495-503 PMID: 3894569
  10. Ecology of Candida albicans gut colonization: inhibition of Candida adhesion, colonization, and dissemination from the gastrointestinal tract by bacterial antagonism.
    Infect Immun. 1985 Sep;49(3):654-63 PMID: 3897061
  11. Effects of carbon dioxide and pH on adhesion of Candida albicans to vaginal epithelial cells.
    Infect Immun. 1985 Oct;50(1):82-90 PMID: 3930406
  12. Adherence of Candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration.
    J Infect Dis. 1985 Dec;152(6):1264-74 PMID: 3905987
  13. Variability in expression of cell surface antigens of Candida albicans during morphogenesis.
    Infect Immun. 1986 Jan;51(1):337-43 PMID: 3510175
  14. Measurement of protein using bicinchoninic acid.
    Anal Biochem. 1985 Oct;150(1):76-85 PMID: 3843705
  15. Adherence of Candida species to host tissues and plastic surfaces.
    Rev Infect Dis. 1986 Jan-Feb;8(1):73-85 PMID: 3513288
  16. Experimental evidence for the role of lipids in adherence of Candida spp. to human buccal epithelial cells.
    Infect Immun. 1986 Oct;54(1):189-93 PMID: 3759234
  17. Correlative relationship between proteinase production, adherence and pathogenicity of various strains of Candida albicans.
    J Med Vet Mycol. 1986 Oct;24(5):407-13 PMID: 3537262
  18. Adhesion of Candida albicans to mouse intestinal mucosa in vitro: development of the assay and test of inhibitors.
    J Med Vet Mycol. 1986 Dec;24(6):477-9 PMID: 3553522
  19. Influence of mucosal cell origin on the in vitro adherence of Candida albicans: are mucosal cells from different sources equivalent?
    Mycopathologia. 1987 May;98(2):111-9 PMID: 3299099
  20. Isolation and partial characterization of an adhesin from Candida albicans.
    J Gen Microbiol. 1987 Mar;133(3):629-36 PMID: 3309163
  21. Adhesion of Candida species to epithelial surfaces.
    Crit Rev Microbiol. 1987;15(1):27-43 PMID: 3319419
  22. Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa.
    Infect Immun. 1988 Mar;56(3):626-31 PMID: 3277916
  23. The physiology and biochemistry of pili.
    Adv Microb Physiol. 1988;29:53-114 PMID: 2898203
  24. Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase.
    Infect Immun. 1988 Aug;56(8):1942-9 PMID: 3294180
  25. Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6157-61 PMID: 3413084
  26. Variation in adhesion and cell surface hydrophobicity in Candida albicans white and opaque phenotypes.
    Mycopathologia. 1988 Jun;102(3):149-56 PMID: 3050525
  27. Participation of yeast cell surface hydrophobicity in adherence of Candida albicans to human epithelial cells.
    Infect Immun. 1989 Jul;57(7):1894-900 PMID: 2659526
  28. Common themes in microbial pathogenicity.
    Microbiol Rev. 1989 Jun;53(2):210-30 PMID: 2569162
  29. Animal glycosphingolipids as membrane attachment sites for bacteria.
    Annu Rev Biochem. 1989;58:309-50 PMID: 2673013
  30. Environmental alteration and phenotypic regulation of Candida albicans adhesion to plastic.
    Infect Immun. 1989 Dec;57(12):3876-81 PMID: 2680985
  31. Adherence of Candida albicans to components of the subendothelial extracellular matrix.
    FEMS Microbiol Lett. 1990 Mar 15;56(3):249-54 PMID: 2187740
  32. Cryptococcus neoformans, Candida albicans, and other fungi bind specifically to the glycosphingolipid lactosylceramide (Gal beta 1-4Glc beta 1-1Cer), a possible adhesion receptor for yeasts.
    Infect Immun. 1990 Jul;58(7):2085-90 PMID: 2194958
  33. Equilibrium analysis of binding of Candida albicans to human buccal epithelial cells.
    Can J Microbiol. 1990 May;36(5):336-40 PMID: 2202504
  34. Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans.
    Infect Immun. 1990 Nov;58(11):3469-76 PMID: 2228219
  35. A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix.
    J Infect Dis. 1991 Mar;163(3):604-10 PMID: 1825316
  36. Differential adherence of hydrophobic and hydrophilic Candida albicans yeast cells to mouse tissues.
    Infect Immun. 1991 Mar;59(3):907-12 PMID: 1900065
  37. Adherence and receptor relationships of Candida albicans.
    Microbiol Rev. 1991 Mar;55(1):1-20 PMID: 2030668
  38. Molecular mimicry in Candida albicans. Role of an integrin analogue in adhesion of the yeast to human endothelium.
    J Clin Invest. 1991 Jun;87(6):1896-902 PMID: 2040685
  39. Pseudomonas aeruginosa exoenzyme S is an adhesion.
    Infect Immun. 1991 Sep;59(9):2859-63 PMID: 1679039
  40. Putative virulence factors of Candida albicans.
    Annu Rev Microbiol. 1991;45:187-218 PMID: 1741614
  41. Fungal adherence to the vascular compartment: a critical step in the pathogenesis of disseminated candidiasis.
    Clin Infect Dis. 1992 Jan;14(1):340-7 PMID: 1571448
  42. Candida albicans adherence to endothelial cells.
    Microvasc Res. 1992 Mar;43(2):218-26 PMID: 1584063
  43. Cell extracts of Candida albicans block adherence of the organisms to endothelial cells.
    Infect Immun. 1992 Aug;60(8):3087-91 PMID: 1386347
  44. Identification of a 58-kilodalton cell surface fibrinogen-binding mannoprotein from Candida albicans.
    Infect Immun. 1992 Oct;60(10):4221-9 PMID: 1398933
  45. Characterization of a fucoside-binding adhesin of Candida albicans.
    Infect Immun. 1992 Nov;60(11):4734-9 PMID: 1398983
  46. Partial characterization of a Candida albicans fimbrial adhesin.
    Infect Immun. 1994 Jul;62(7):2834-42 PMID: 8005673
  47. Suppression of Candida albicans by human oral streptococci in gnotobiotic mice.
    Infect Immun. 1973 Nov;8(5):846-9 PMID: 4584056
  48. Fungal fimbriae. I. Structure, origin, and synthesis.
    Can J Microbiol. 1975 Apr;21(4):537-46 PMID: 47260
  49. Adherence of Candida albicans to human buccal epithelial cells.
    Infect Immun. 1978 Jul;21(1):64-8 PMID: 361571
  50. Adherence of Candida albicans to a fibrin-platelet matrix formed in vitro.
    Infect Immun. 1980 Feb;27(2):650-6 PMID: 6991422
  51. Adherence of Candida albicans and other Candida species to mucosal epithelial cells.
    Infect Immun. 1980 Feb;27(2):667-74 PMID: 6991423
  52. Relationship between cell surface composition of Candida albicans and adherence to acrylic after growth on different carbon sources.
    Infect Immun. 1981 Jun;32(3):1234-41 PMID: 7019091
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.7.2843-2848.1994
Published
1994-07-00
Pages
2843-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC302890
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.62.7.2843-2848.1994 PMC Full Text View journal details
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