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PMID: 8005673 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Partial characterization of a Candida albicans fimbrial adhesin.

Infection and immunity ·Vol. 62 ·No. 7 ·1994-07-00 ·Pages 2834-42

Yu L, Lee KK, Ens K, Doig PC, Carpenter MR, Staddon W, Hodges RS, Paranchych W, Irvin RT

Abstract

Candida albicans is the primary etiologic agent of candidiasis, a disease that can vary from superficial mucosal lesions to life-threatening systemic or disseminated diseases. Strains of C. albicans have been reported to possess long, thin filamentous protein cell surface appendages termed fimbriae (R.B. Gardiner, M. Canton, and A. W. Day, Bot. Gaz. 143:534-541, 1982). These fimbriae were isolated, purified, and partially characterized. The major structural subunit of the fimbriae is a glycoprotein which consists of 80 to 85% carbohydrate (consisting primarily of D-mannose) and 10 to 15% protein. The molecular weight of the glycosylated fimbrial subunit is approximately 66,000, while unglycosylated protein has an approximate molecular weight of 8,644. The fimbriae function as adhesins mediating C. albicans binding to human buccal epithelial cells. Amino acid analysis of the purified fimbrial subunit indicates that the fimbrial subunit is composed of 50% hydrophobic amino acid residues. The N terminus of the fimbrial subunit is blocked to N-terminal sequencing.

MeSH Terms
Amino Acids/analysis Antibodies, Fungal Antibodies, Monoclonal Candida albicans/chemistry,immunology,ultrastructure Cell Adhesion Cell Adhesion Molecules/chemistry,immunology Epithelial Cells Epithelium/microbiology Fungal Proteins/chemistry,immunology Glycoproteins/chemistry,immunology Humans Male Mouth/cytology,microbiology Organelles/chemistry,immunology,ultrastructure
Chemicals
Amino Acids Antibodies, Fungal Antibodies, Monoclonal Cell Adhesion Molecules Fungal Proteins Glycoproteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Yu L
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Lee K K
Ens K
Doig P C
Carpenter M R
Staddon W
Hodges R S
Paranchych W
Irvin R T
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.7.2834-2842.1994
Published
1994-07-00
Pages
2834-42
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC302889
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.62.7.2834-2842.1994 PMC Full Text View journal details
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