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PMID: 8002606 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation of a Bacillus subtilis spoIIGA allele that suppresses processing-negative mutations in the Pro-sigma E gene (sigE).

Journal of bacteriology ·Vol. 176 ·No. 24 ·1994-12-00 ·Pages 7763-6

Peters HK, Haldenwang WG

Abstract

sigma E, a sporulation-essential sigma factor of Bacillus subtilis, is formed by a developmentally regulated proteolysis which removes 27 to 29 amino acids from the amino terminus of an inactive precursor protein (Pro-sigma E). A mutation which facilitates the conversion of inefficiently processed Pro-sigma E variants into mature sigma E was identified and mapped to spoIIGA. The isolation of such a mutation argues that SpoIIGA is directly involved in the Pro-sigma E processing reaction.

MeSH Terms
Alleles Amino Acid Sequence Bacillus subtilis/genetics,growth & development,metabolism Bacterial Proteins/biosynthesis,genetics Membrane Proteins/genetics Molecular Sequence Data Mutation Peptide Hydrolases Protein Processing, Post-Translational Recombinant Fusion Proteins/biosynthesis Sequence Homology, Amino Acid Sigma Factor Spores, Bacterial/growth & development Suppression, Genetic Transcription Factors
Chemicals
Bacterial Proteins Membrane Proteins Recombinant Fusion Proteins Sigma Factor Transcription Factors sporulation-specific sigma factors Peptide Hydrolases SpoIIGA protein, Bacillus subtilis
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peters H K
Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758.
Haldenwang W G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.24.7763-7766.1994
Published
1994-12-00
Pages
7763-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197240
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.24.7763-7766.1994 PMC Full Text View journal details
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