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PMID: 2448286 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation.

Journal of bacteriology ·Vol. 170 ·No. 2 ·1988-02-00 ·Pages 507-11

Jonas RM, Weaver EA, Kenney TJ, Moran CP, Haldenwang WG

Abstract

A sporulation-specific sigma factor of Bacillus subtilis (sigma E) is formed by a proteolytic activation of a precursor protein (P31). Synthesis of the precursor protein is shown to be abolished in B. subtilis mutants with plasmid insertions as far as 940 base pairs upstream of the P31 structural gene (sigE), and processing of P31 to sigma E is blocked by a deletion in this upstream region. These results substantiate the view that sigE is the distal member of a 2-gene operon and demonstrate that the upstream gene (spoIIGA) is necessary for sigma E formation.

MeSH Terms
Alleles Bacillus subtilis/genetics,metabolism,physiology Gene Expression Regulation Genes Genes, Bacterial Mutation Nucleic Acid Hybridization Operon Plasmids Promoter Regions, Genetic Protein Precursors/biosynthesis,genetics Protein Processing, Post-Translational RNA, Bacterial/genetics Sigma Factor/biosynthesis,genetics Spores, Bacterial Transcription Factors/genetics Transcription, Genetic Transformation, Bacterial
Chemicals
Protein Precursors RNA, Bacterial Sigma Factor Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jonas R M
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
Weaver E A
Kenney T J
Moran C P
Haldenwang W G
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18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.2.507-511.1988
Published
1988-02-00
Pages
507-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210682
Subset
IM
Grants
NIAID NIH HHS · AI20319 · United States
External Links
PubMed source DOI: 10.1128/jb.170.2.507-511.1988 PMC Full Text View journal details
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