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PMID: 8002570 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 176 ·No. 24 ·1994-12-00 ·Pages 7476-83

Coffman JA, el Berry HM, Cooper TG

Abstract

Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR element and trans-acting GLN3. Here, we extend the analysis to include the response of their expression to deletion of the URE2 locus. The expression of these nitrogen catabolic genes becomes, to various degrees, NCR insensitive in the ure2 deletion. This response is shown to be mediated through the GATAA-containing UASNTR element and supports the current idea that the NCR regulatory circuit involves the following steps: environmental signal-->URE2-->GLN3-->UASNTR operation-->NCR-sensitive gene expression. The various responses of the nitrogen catabolic genes' expression to deletion of the URE2 locus also indicate that not all NCR is mediated through URE2.

MeSH Terms
Allantoin/metabolism Amino Acids/metabolism Base Sequence Biological Transport/genetics Fungal Proteins/genetics Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal/genetics Genes, Reporter Glutathione Peroxidase Molecular Sequence Data Nitrogen/metabolism Prions Proline/metabolism Promoter Regions, Genetic/genetics Regulatory Sequences, Nucleic Acid/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription, Genetic gamma-Aminobutyric Acid/metabolism
Chemicals
Amino Acids Fungal Proteins Prions Saccharomyces cerevisiae Proteins Allantoin gamma-Aminobutyric Acid Proline Glutathione Peroxidase URE2 protein, S cerevisiae Nitrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Coffman J A
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
el Berry H M
Cooper T G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.24.7476-7483.1994
Published
1994-12-00
Pages
7476-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197203
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-35642 · United States
External Links
PubMed source DOI: 10.1128/jb.176.24.7476-7483.1994 PMC Full Text View journal details
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