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PMID: 8045902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 176 ·No. 15 ·1994-08-00 ·Pages 4718-25

Cunningham TS, Dorrington RA, Cooper TG

Abstract

Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the gamma-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA-containing sequences (URSGATA) in vitro, and a pair of GATA-containing sequences (UASNTR) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.

Related Genes
GlN3 UGA1 UGA4
MeSH Terms
4-Aminobutyrate Transaminase/genetics Allantoin/metabolism Base Sequence Carrier Proteins/genetics DNA-Binding Proteins/metabolism Fungal Proteins/genetics,metabolism GABA Plasma Membrane Transport Proteins GATA Transcription Factors Gene Expression Regulation, Fungal Membrane Transport Proteins/genetics,metabolism Molecular Sequence Data Organic Anion Transporters Protein Binding Regulatory Sequences, Nucleic Acid/genetics Repressor Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic gamma-Aminobutyric Acid/metabolism
Chemicals
Carrier Proteins DAL80 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins GATA Transcription Factors GLN3 protein, S cerevisiae Membrane Transport Proteins Organic Anion Transporters Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors UGA4 protein, S cerevisiae Allantoin gamma-Aminobutyric Acid 4-Aminobutyrate Transaminase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cunningham T S
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Dorrington R A
Cooper T G
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.15.4718-4725.1994
Published
1994-08-00
Pages
4718-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196294
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-35642 · United States
Databases
GENBANK
L28080, L28081
External Links
PubMed source DOI: 10.1128/jb.176.15.4718-4725.1994 PMC Full Text View journal details
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