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PMID: 7983758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription factor Oct1 binds to the AT-rich segment of the simian virus 40 replication origin.

Journal of virology ·Vol. 69 ·No. 1 ·1995-01-00 ·Pages 575-8

Kilwinski J, Baack M, Heiland S, Knippers R

Abstract

A cellular protein that binds to the AT-rich late segment of the simian virus 40 (SV40) origin of replication has been identified as transcription factor Oct1. This conclusion is based on the following observations: the late origin binding protein has a molecular mass of about 100 kDa, like factor Oct1, and shares other biochemical properties with Oct1; its binding to the origin is inhibited by antibodies directed against the POU domain of factor Oct1; the isolated POU domain of Oct1 specifically binds to the SV40 late origin region. Thus, the SV40 genome contains binding sites for transcription factor Oct1 in the origin of replication in addition to the previously characterized octamer sites in the viral promoter enhancer. Oct1, bound to the viral origin, negatively affects the DNA unwinding reaction catalyzed by the viral replication initiator T antigen, suggesting that Oct1 may have a role in the regulation of viral replication.

MeSH Terms

Antigens, Polyomavirus Transforming/metabolism Base Sequence Binding Sites Catalysis DNA-Binding Proteins/metabolism HeLa Cells Host Cell Factor C1 Humans Molecular Sequence Data Octamer Transcription Factor-1 Replication Origin Sequence Homology, Nucleic Acid Simian virus 40/genetics,metabolism Transcription Factors/metabolism

Chemicals

Antigens, Polyomavirus Transforming DNA-Binding Proteins HCFC1 protein, human Host Cell Factor C1 Octamer Transcription Factor-1 POU2F1 protein, human Transcription Factors

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kilwinski J

Division of Biology, Universität Konstanz, Federal Republic of Germany.

Baack M

Heiland S

Knippers R

References (24)

24 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.69.1.575-578.1995

Published

1995-01-00

Pages

575-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC188613

Subset

External Links

PubMed source DOI: 10.1128/JVI.69.1.575-578.1995 PMC Full Text View journal details

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Transcription factor Oct1 binds to the AT-rich segment of the simian virus 40 replication origin.

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