Home LiteratureArticle Details
PMID: 7972064 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microtubule dynamics in vitro are regulated by the tubulin isotype composition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 24 ·1994-11-22 ·Pages 11358-62

Panda D, Miller HP, Banerjee A, Ludueña RF, Wilson L

Abstract

The growing and shortening dynamics of individual bovine brain microtubules at their plus ends at steady state in vitro, assembled from isotypically pure alpha beta II, alpha beta III, or alpha beta IV tubulin dimers, were determined by differential interference contrast video microscopy. Microtubules assembled from the purified alpha beta III isotype were considerably more dynamic than microtubules made from the alpha beta II or alpha beta IV isotypes or from unfractionated phosphocellulose-purified tubulin. Furthermore, increasing the proportion of the alpha beta II isotype in a mixture of the alpha beta II and alpha beta III isotypes suppressed microtubule dynamics, demonstrating that microtubule dynamics can be influenced by the tubulin isotype composition. The data support the hypothesis that cells might determine the dynamic properties and functions of its microtubules in part by altering the relative amounts of the different tubulin isotypes.

MeSH Terms
Animals Brain Chemistry Cattle In Vitro Techniques Kinetics Microtubules/physiology Protein Binding Tubulin/classification,physiology
Chemicals
Tubulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Panda D
Department of Biological Sciences, University of California, Santa Barbara 93106.
Miller H P
Banerjee A
Ludueña R F
Wilson L
References (30)
30 references, click to expand
  1. Characterization of posttranslational modifications in neuron-specific class III beta-tubulin by mass spectrometry.
    Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4685-9 PMID: 2052551
  2. Distinct colchicine binding kinetics of bovine brain tubulin lacking the type III isotype of beta-tubulin.
    J Biol Chem. 1991 Jan 25;266(3):1689-91 PMID: 1988443
  3. Microtubules grow and shorten at intrinsically variable rates.
    J Biol Chem. 1992 Apr 25;267(12):7995-8006 PMID: 1569058
  4. Kinetics of colchicine binding to purified beta-tubulin isotypes from bovine brain.
    J Biol Chem. 1992 Jul 5;267(19):13335-9 PMID: 1618835
  5. Differential sorting of beta tubulin isotypes into colchicine-stable microtubules during neuronal and muscle differentiation of embryonal carcinoma cells.
    Cell Motil Cytoskeleton. 1992;21(4):313-25 PMID: 1628327
  6. Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis.
    J Cell Sci. 1992 Jul;102 ( Pt 3):401-16 PMID: 1506423
  7. Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific.
    J Cell Biol. 1993 Feb;120(4):935-45 PMID: 8432733
  8. Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine.
    Biochemistry. 1993 Feb 9;32(5):1285-93 PMID: 8448138
  9. A functionally specialized alpha-tubulin is required for oocyte meiosis and cleavage mitoses in Drosophila.
    Development. 1993 Mar;117(3):977-91 PMID: 8325246
  10. Are tubulin isotypes functionally significant.
    Mol Biol Cell. 1993 May;4(5):445-57 PMID: 8334301
  11. In vitro analysis of microtubule assembly of isotypically pure tubulin dimers. Intrinsic differences in the assembly properties of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers in the absence of microtubule-associated proteins.
    J Biol Chem. 1994 Jan 21;269(3):2041-7 PMID: 8294455
  12. Quantification of microtubule dynamics in living plant cells using fluorescence redistribution after photobleaching.
    J Cell Sci. 1994 Apr;107 ( Pt 4):775-84 PMID: 8056836
  13. [Biochemical control of recombination in Saccharomyces cerevisiae. I. L-histidine inhibition of intragenic mitotic recombination at the ad 3 locus].
    Mol Gen Genet. 1971;111(2):120-37 PMID: 5564465
  14. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  15. Opposite end assembly and disassembly of microtubules at steady state in vitro.
    Cell. 1978 Jan;13(1):1-8 PMID: 620419
  16. Microtubule treadmills--possible molecular machinery.
    Nature. 1981 Oct 29;293(5835):705-11 PMID: 7027052
  17. Involvement of the carboxyl-terminal domain of tubulin in the regulation of its assembly.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5989-93 PMID: 6385005
  18. Tubulin dynamics in cultured mammalian cells.
    J Cell Biol. 1984 Dec;99(6):2175-86 PMID: 6501419
  19. Microtubule assembly nucleated by isolated centrosomes.
    Nature. 1984 Nov 15-21;312(5991):232-7 PMID: 6504137
  20. Identification of conserved isotype-defining variable region sequences for four vertebrate beta tubulin polypeptide classes.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4327-31 PMID: 3459176
  21. Common and distinct tubulin binding sites for microtubule-associated proteins.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7162-6 PMID: 3463956
  22. Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.
    Mol Cell Biol. 1986 Jul;6(7):2409-19 PMID: 3785200
  23. Phase dynamics at microtubule ends: the coexistence of microtubule length changes and treadmilling.
    J Cell Biol. 1987 Apr;104(4):1035-46 PMID: 3558477
  24. Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
    J Cell Biol. 1988 Oct;107(4):1437-48 PMID: 3170635
  25. Dynamics of microtubules visualized by darkfield microscopy: treadmilling and dynamic instability.
    Cell Motil Cytoskeleton. 1988;10(1-2):229-36 PMID: 2972399
  26. MAP-1 and MAP-2 binding sites at the C-terminus of beta-tubulin. Studies with synthetic tubulin peptides.
    Biochemistry. 1991 Apr 30;30(17):4362-6 PMID: 2021628
  27. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin.
    J Biol Chem. 1990 Jan 25;265(3):1794-9 PMID: 2404018
  28. Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
    Cell. 1990 Aug 10;62(3):579-89 PMID: 2379239
  29. Two Drosophila beta tubulin isoforms are not functionally equivalent.
    J Cell Biol. 1990 Sep;111(3):1009-26 PMID: 2118141
  30. Preparation of a monoclonal antibody specific for the class IV isotype of beta-tubulin. Purification and assembly of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers from bovine brain.
    J Biol Chem. 1992 Mar 15;267(8):5625-30 PMID: 1544937
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.24.11358
Published
1994-11-22
Pages
11358-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45230
Subset
IM
Grants
NCI NIH HHS · CA26376 · United States
NIGMS NIH HHS · GM23476 · United States
NINDS NIH HHS · NS13560 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.24.11358 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2