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PMID: 7966248 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A chloride channel reconstituted from fetal rat brain growth cones.

The Journal of membrane biology ·Vol. 141 ·No. 1 ·1994-07-00 ·Pages 7-19

DeBin JA, Wood MR, Pfenninger KH, Strichartz GR

Abstract

Chloride channels were reconstituted into planar lipid bilayers isolated from a preparation of growth cone particles (GCPs) isolated from fetal rat brain. One type of channel was predominantly seen and some of its biophysical and pharmacological properties were studied. The single channel i-V relationship was curvilinear with a chord conductance of 75 pS at +30 mV in symmetric 200 mM NaCl solutions buffered with phosphate. The channel was inactivated by depolarization, and this inactivation was reversed rapidly upon returning to -25 mV. The Cl- channel was significantly permeant to Na+ ions (PNa/PCl = 0.26), and the relative halide permeabilities were determined to be: I(1.92) > Br(1.73) > Cl(1.0) > F(0.34). The channel was inhibited by the common stilbene compounds (DIDS, SITS, DNDS), as well as by Zn2+ ions and an indanyloxyacetic acid derivative. A developmental role for the GCP Cl- channel is suggested by the observation that adult rat brain synaptosomal membranes were nearly devoid of this type of Cl- channel.

MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/pharmacology Animals Brain/cytology Cell Membrane/physiology Chloride Channels/drug effects,isolation & purification,physiology Membranes, Artificial Rats Retinal Cone Photoreceptor Cells/cytology Stilbenes/pharmacology Synaptosomes/physiology
Chemicals
Chloride Channels Membranes, Artificial Stilbenes 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 4,4'-dinitro-2,2'-stilbenedisulfonic acid 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
DeBin J A
Anesthesia Research Laboratories, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Wood M R
Pfenninger K H
Strichartz G R
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF00232869
Published
1994-07-00
Pages
7-19
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIGMS NIH HHS · GM15904 · United States
NINDS NIH HHS · NS24676 · United States
External Links
PubMed source DOI: 10.1007/BF00232869 View journal details
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