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PMID: 7949421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of DLC-A and DLC-B, two families of cytoplasmic dynein light chain subunits.

Molecular biology of the cell ·Vol. 5 ·No. 6 ·1994-06-00 ·Pages 645-54

Gill SR, Cleveland DW, Schroer TA

Abstract

Cytoplasmic dynein is a minus-end-directed, microtubule-dependent motor composed of two heavy chains (approximately 530 kDa), three intermediate chains (approximately 74 kDa), and a family of approximately 52-61 kDa light chains. Although the approximately 530 kDa subunit contains the motor and microtubule binding domains of the complex, the functions of the smaller subunits are not known. Using two-dimensional gel electrophoresis and proteolytic mapping, we show here that the light chains are composed of two major families, a higher M(r) family (58, 59, 61 kDa; dynein light chain group A [DLC-A]) and lower M(r) family (52, 53, 55, 56 kDa; dynein light chain group B [DLC-B]). Dissociation of the cytoplasmic dynein complex with potassium iodide reveals that all light chain polypeptides are tightly associated with the approximately 530 kDa heavy chain, whereas the approximately 74 kDa intermediate chain polypeptides are more readily extracted. Treatment with alkaline phosphatase alters the mobility of four of the light chain polypeptides, indicating that these subunits are phosphorylated. Sequencing of a cDNA clone encoding one member of the DLC-A family reveals a predicted globular structure that is not homologous to any known protein but does contain numerous potential phosphorylation sites and a consensus nucleotide-binding motif.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites/genetics Brain/enzymology Cattle Chick Embryo Cloning, Molecular DNA Primers/genetics DNA, Complementary/genetics Dyneins/chemistry,genetics Molecular Sequence Data Molecular Weight Peptide Mapping Phosphorylation Protein Conformation
Chemicals
DNA Primers DNA, Complementary Dyneins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gill S R
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
Cleveland D W
Schroer T A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.5.6.645
Published
1994-06-00
Pages
645-54
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301080
Subset
IM
Databases
GENBANK
X79088
External Links
PubMed source DOI: 10.1091/mbc.5.6.645 PMC Full Text View journal details
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