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PMID: 7948905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dehydration and ABA increase mRNA levels and enzyme activity of cytosolic GAPDH in the resurrection plant Craterostigma plantagineum.

Plant molecular biology ·Vol. 26 ·No. 1 ·1994-10-00 ·Pages 541-6

Velasco R, Salamini F, Bartels D

Abstract

We report the identification of a full-length cDNA clone encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in the desiccation-tolerant plant Craterostigma plantagineum. The DNA sequence of the cDNA clone is homologous to cytosolic GAPDH cDNAs from other higher plants. The GAPDH transcript increases rapidly in abundance during dehydration or abscisic acid (ABA) treatment. The increase in mRNA levels is directly correlated with higher protein and enzyme levels. These results imply that enhanced rates of glycolysis are one of the immediate cellular responses to water deficit. This may be a mechanism by which the plant cell prepares for a demand of ATP and NADH2 during recovery.

MeSH Terms

Abscisic Acid/pharmacology Amino Acid Sequence Cloning, Molecular Cytosol/metabolism DNA, Complementary/genetics Desiccation Gene Expression Regulation, Plant/drug effects,physiology Gene Library Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis,genetics,metabolism Molecular Sequence Data Plants/enzymology,genetics RNA, Messenger/analysis,biosynthesis RNA, Plant/analysis Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid

Chemicals

DNA, Complementary RNA, Messenger RNA, Plant Abscisic Acid Glyceraldehyde-3-Phosphate Dehydrogenases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Velasco R

Max-Planck-Institut für Züchtungsforschung, Köln, Germany.

Salamini F

Bartels D

References (21)

21 references, click to expand

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00039567

Published

1994-10-00

Pages

541-6

Language

English

Region

Netherlands

NLM ID

9106343

Subset

Databases

GENBANK

X78307

External Links

PubMed source DOI: 10.1007/BF00039567 View journal details

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Dehydration and ABA increase mRNA levels and enzyme activity of cytosolic GAPDH in the resurrection plant Craterostigma plantagineum.

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