Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase contains two subunits differentially regulated by anaerobiosis.

Plant molecular biology ·Vol. 12 ·No. 2 ·1989-02-00 ·Pages 131-9

Ricard B, Rivoal J, Pradet A

Abstract

Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is composed of two subunits of different molecular weights. Cytosolic GAPDH activity and protein both decreased immediately after transfer of 48-h rice seedlings to anaerobic conditions. Subsequent increase in activity and protein was accompanied by a change in isoenzyme profile and was preceded by an increase in steady-state messenger levels. One and two-dimensional electrophoretic analyses of in vivo and in vitro labeled GAPDH suggested that the change in isoenzyme profile under anaerobic conditions is due to preferential synthesis of one of the two GAPDH subunits caused by a specific increase in its mRNA.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ricard B

Station de Physiologie Végétale, I.N.R.A., 33140, Pont-de-la Maye, France.

Rivoal J

Pradet A

References (14)

14 references, click to expand

DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene.
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6624-8 PMID: 16578816
Evolutionary divergence of chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from angiosperms.
Eur J Biochem. 1982 Sep 1;126(3):513-5 PMID: 7140744
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
Cell. 1977 Dec;12(4):1133-41 PMID: 23215
Rice embryos can express heat-shock genes under anoxia.
Biochimie. 1987 Jun-Jul;69(6-7):677-81 PMID: 2446669
Quaternary structure of higher plant glyceraldehyde-3-phosphate dehydrogenases.
Eur J Biochem. 1979 Feb 15;94(1):243-7 PMID: 35350
Adaptation of ribonucleic Acid metabolism to anoxia in rice embryos.
Plant Physiol. 1983 May;72(1):115-21 PMID: 16662943
Two isoenzymes of glyceraldehyde-3-phosphate dehydrogenase in Caenorhabditis elegans. Isolation, properties, and immunochemical characterization.
J Biol Chem. 1984 Dec 10;259(23):14711-20 PMID: 6389551
Effect of anoxia on energy charge and protein synthesis in rice embryo.
Plant Physiol. 1981 Sep;68(3):636-40 PMID: 16661971
Cat-2 gene expression. Developmental control of translatable CAT-2 mRNA levels in maize scutellum.
Mol Gen Genet. 1986 Apr;203(1):185-8 PMID: 2423849
Expression of alcohol dehydrogenase in rice embryos under anoxia.
Plant Mol Biol. 1986 Sep;7(5):321-9 PMID: 24302402
The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein.
Mol Gen Genet. 1986 Dec;205(3):461-8 PMID: 2436026
Endosymbiotic origin and codon bias of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize.
J Mol Evol. 1987;26(4):320-8 PMID: 3131533
Subunit structure of higher plant glyceraldehyde-3-phosphate dehydrogenases (EC 1.2.1.12 and EC 1.2.1.13).
J Biol Chem. 1979 Jul 10;254(13):6094-8 PMID: 109446
Maize Adh1.
Annu Rev Genet. 1985;19:297-323 PMID: 3002240

Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00020498

Published

1989-02-00

Pages

131-9

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00020498 View journal details

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Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase contains two subunits differentially regulated by anaerobiosis.

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