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PMID: 7933072 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a Borna disease virus glycoprotein, gp18.

Journal of virology ·Vol. 68 ·No. 11 ·1994-11-00 ·Pages 6918-23

Kliche S, Briese T, Henschen AH, Stitz L, Lipkin WI

Abstract

Borna disease virus is a nonsegmented negative-strand RNA virus that causes neurologic disease in a wide variety of animal hosts. Here we describe identification and characterization of the first glycoprotein in this viral system. The 18-kDa glycoprotein, gp18, has been purified from infected rat brain. Isolation and microsequencing of this protein allowed identification of a 16.2-kDa open reading frame in the viral antigenome. Lectin binding and endoglycosidase sensitivity assays indicate that gp18 is an unusual N-linked glycoprotein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Borna disease virus/chemistry Glycoproteins/biosynthesis,chemistry,isolation & purification Mice Mice, Inbred BALB C Molecular Sequence Data Rats Rats, Inbred Lew Viral Proteins/biosynthesis,chemistry,isolation & purification
Chemicals
Glycoproteins Viral Proteins gp18 protein, Borna disease virus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kliche S
Department of Neurology, University of California, Irvine 92717.
Briese T
Henschen A H
Stitz L
Lipkin W I
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.11.6918-6923.1994
Published
1994-11-00
Pages
6918-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237127
Subset
IM
Grants
NINDS NIH HHS · NS-29425 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.11.6918-6923.1994 PMC Full Text View journal details
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