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PMID: 7929016 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gene fusion analysis of membrane protein topology: a direct comparison of alkaline phosphatase and beta-lactamase fusions.

Journal of bacteriology ·Vol. 176 ·No. 20 ·1994-10-00 ·Pages 6410-3

Prinz WA, Beckwith J

Abstract

To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to beta-lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent beta-lactamase fusions do not. This disparity may reflect differences in the folding of beta-lactamase and alkaline phosphatase in the cytoplasm.

MeSH Terms
Alkaline Phosphatase/chemistry,genetics Amino Acid Sequence Bacterial Proteins/chemistry,genetics Carrier Proteins/chemistry,genetics Cell Membrane Cloning, Molecular Escherichia coli Proteins Maltose-Binding Proteins Membrane Proteins/chemistry,genetics Membrane Transport Proteins/chemistry,genetics Molecular Sequence Data Monosaccharide Transport Proteins Protein Conformation Recombinant Fusion Proteins/chemistry,genetics Symporters beta-Lactamases/chemistry,genetics
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins LacY protein, E coli Maltose-Binding Proteins Membrane Proteins Membrane Transport Proteins Monosaccharide Transport Proteins Recombinant Fusion Proteins Symporters lactose permease Alkaline Phosphatase beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prinz W A
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Beckwith J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.20.6410-6413.1994
Published
1994-10-00
Pages
6410-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196986
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.20.6410-6413.1994 PMC Full Text View journal details
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