Home LiteratureArticle Details

PMID: 7906933 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization.

Applied and environmental microbiology ·Vol. 60 ·No. 1 ·1994-01-00 ·Pages 377-84

Berthier Y, Thierry D, Lemattre M, Guesdon JL

Abstract

A new insertion sequence was isolated from Xanthomonas campestris pv. dieffenbachiae. Sequence analysis showed that this element is 1,158 bp long and has 15-bp inverted repeat ends containing two mismatches. Comparison of this sequence with sequences in data bases revealed significant homology with Escherichia coli IS5. IS1051, which detected multiple restriction fragment length polymorphisms, was used as a probe to characterize strains from the pathovar dieffenbachiae.

MeSH Terms

Base Sequence DNA Primers/genetics DNA Probes DNA Transposable Elements DNA, Bacterial/genetics Enterobacteriaceae/genetics,isolation & purification Escherichia coli/genetics Molecular Sequence Data Plants/microbiology Polymorphism, Restriction Fragment Length Pseudomonadaceae/genetics,isolation & purification Sequence Homology, Nucleic Acid Species Specificity Xanthomonas campestris/classification,genetics,isolation & purification

Chemicals

DNA Primers DNA Probes DNA Transposable Elements DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Berthier Y

Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, France.

Thierry D

Lemattre M

Guesdon J L

References (9)

9 references, click to expand

IS6110, an IS-like element of Mycobacterium tuberculosis complex.
Nucleic Acids Res. 1990 Jan 11;18(1):188 PMID: 2155396
The nucleotide sequence and protein-coding capability of the transposable element IS5.
Gene. 1981 Aug;14(3):155-63 PMID: 6269958
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Assessment of genetic diversity and population structure of Xanthomonas oryzae pv. oryzae with a repetitive DNA element.
Appl Environ Microbiol. 1992 Jul;58(7):2188-95 PMID: 1353345
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Characterization of IS476 and its role in bacterial spot disease of tomato and pepper.
J Bacteriol. 1990 Jan;172(1):143-8 PMID: 2152895
Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.
J Clin Microbiol. 1993 May;31(5):1048-54 PMID: 8501206
Nucleotide sequence and characterization of a repetitive DNA element from the genome of Bordetella pertussis with characteristics of an insertion sequence.
J Gen Microbiol. 1988 Aug;134(8):2297-306 PMID: 2908119
Characterization of Xanthomonas campestris Pathovars by rRNA Gene Restriction Patterns.
Appl Environ Microbiol. 1993 Mar;59(3):851-9 PMID: 16348894

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.60.1.377-384.1994

Published

1994-01-00

Pages

377-84

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC201318

Subset

Databases

GENBANK

X70380

External Links

PubMed source DOI: 10.1128/aem.60.1.377-384.1994 PMC Full Text View journal details

Analysis Services

Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization.

Contact

WeChat Official Account