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PMID: 790151 Published · ppublish English Journal Article

Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation.

Molecular & general genetics : MGG ·Vol. 148 ·No. 2 ·1976-10-18 ·Pages 125-30

Defais M, Caillet-Fauquet P, Fox MS, Radman M

Abstract

Ultraviolet mutagenesis of phage gamma is produced by host functions which are inducible by ultraviolet irradiation of the host cell. Induction kinetics and the half life of the inducible mutagenic DNA repair (SOS-repair) in E.coli have been determined using phage gamma assays. At 37 degrees C, both mutagenic and repair activities are maximal approximately 30 min following irradiation and decay with a half life of approximately 30 min. The presence of 100 mug/ml chloramphenicol during the first 40 min after irradiation completely abolishes induction of repair and mutagenesis. The ultraviolet induction pattern of SOS repair very much resembles that of gamma prophage in lysogenic induction (Monk and Kinross, 1975).

MeSH Terms
Chloramphenicol/pharmacology Coliphages/radiation effects DNA Repair Depression, Chemical Escherichia coli/drug effects,radiation effects Kinetics Mutation/drug effects Ultraviolet Rays
Chemicals
Chloramphenicol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Defais M
Caillet-Fauquet P
Fox M S
Radman M
References (20)
20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268375
Published
1976-10-18
Pages
125-30
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268375
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