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PMID: 1105587 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of error-prone repair as a consequence of DNA ligase deficiency in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4645-9

Morse LS, Pauling C

Abstract

DNA ligase deficiency is shown to induce generalized mutator activity in E. coli. This mutator activity is unaffected by 3 mug/ml of chloramphenicol but is abolished both in lig-recA double mutants and by incubation with 20 mug/ml of chloramphenicol. Dna ligase deficiency is also shown to reactivate ultraviolet light-irradiated phage lambda and T7 and to increase both spontaneous and ultraviolet light-induced mutagenesis in phage lambda, all of which are abolished in lig-recA strains. Interaction occurs between the molecular events of mutagenesis induced by ultraviolet irradiation and those induced by DNA ligase deficiency. These observations suggest a common pathway, coordinately expressed with the inducible repair mode, that is responsible for mutagenesis.

MeSH Terms
Chloramphenicol/pharmacology Coliphages/radiation effects DNA Repair Dose-Response Relationship, Radiation Escherichia coli/enzymology,radiation effects Mutation/drug effects Polynucleotide Ligases/metabolism Radiation Effects Temperature Ultraviolet Rays
Chemicals
Chloramphenicol Polynucleotide Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morse L S
Pauling C
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4645
Published
1975-11-00
Pages
4645-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388780
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4645 PMC Full Text View journal details
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