Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication.
We have described a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. In vitro assays have demonstrated that DNA ligase activity is markedly reduced in this strain relative to that of the parent strain. Okazaki fragments of newly replicated DNA accumulate in this mutant strain upon growth at the restrictive temperature. These results imply that the same enzyme, DNA ligase, functions both in normal semiconservative replication and in nonconservative repair replication of DNA.
8 references, click to expand
-
Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.Proc Natl Acad Sci U S A. 1967 May;57(5):1426-33 PMID: 5341238
-
Formation of covalent circles of lambda DNA by E. coli extracts.Proc Natl Acad Sci U S A. 1967 Jan;57(1):148-55 PMID: 4860192
-
Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605 PMID: 4967086
-
Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495-502 PMID: 4877274
-
Intermediates in T4 DNA replication in a T4 ligase deficient strain.Cold Spring Harb Symp Quant Biol. 1968;33:145-50 PMID: 4891959
-
Involvement of synthesis of RNA in thymineless death.Nature. 1963 Apr 20;198:286 PMID: 13952474
-
ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOME.Proc Natl Acad Sci U S A. 1964 Jul;52:125-32 PMID: 14192649
-
EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA.J Mol Biol. 1964 Aug;9:395-410 PMID: 14202275
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
